DNA barcode assessment of Ceramiales (Rhodophyta) in the intertidal zone of the northwestern Yellow Sea

Guoying Du; Feifei Wu; Hao Guo; Hongfan Xue; Yunxiang Mao

doi:10.1007/s00343-015-4088-8

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DNA barcode assessment of Ceramiales (Rhodophyta) in the intertidal zone of the northwestern Yellow Sea

Updated：2023-05-16

- DNA barcode assessment of Ceramiales (Rhodophyta) in the intertidal zone of the northwestern Yellow Sea
- Journal of Oceanology and Limnology Vol. 33, Issue 3, Pages: 685-695(2015)
- Affiliations：
  
  1. Bioengineering Department College of Marine Life Science Ocean University of China
  2. National Marine Environmental Monitoring Center
- Author bio：
- Funds：
- DOI：10.1007/s00343-015-4088-8
  CLC：
- Online First：16 January 2015，
  
  Published：2015
- Accepted：
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Du, G., Wu, F., Guo, H. et al. DNA barcode assessment of Ceramiales (Rhodophyta) in the intertidal zone of the northwestern Yellow Sea., Chin. J. Ocean. Limnol. 33, 685–695 (2015). https://doi.org/10.1007/s00343-015-4088-8
DOI：

Du, G., Wu, F., Guo, H. et al. DNA barcode assessment of Ceramiales (Rhodophyta) in the intertidal zone of the northwestern Yellow Sea., Chin. J. Ocean. Limnol. 33, 685–695 (2015). https://doi.org/10.1007/s00343-015-4088-8 DOI：

摘要

Abstract

A total of 142 specimens of Ceramiales (Rhodophyta) were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species

14 genera

and four families. Cluster analyses show that the specimens had a high diversity for the three DNA markers

namely

partial large subunit rRNA gene (LSU)

universal plastid amplicon (UPA)

and partial mitochondrial cytochrome c oxidase subunit I gene (COI). No intraspecific divergence was found in our collection for these markers

except for a 1–3 bp divergence in the COI of Ceramium kondoi

Symphyocladia latiuscula

and Neosiphonia japonica. Because short DNA markers were used

the phylogenetic relationships of higher taxonomic levels were hard to evaluate with poor branch support. More than half species of our collection failed to find their matched sequences owing to shortage information of DNA barcodes for macroalgae in GenBank or BOLD (Barcode of Life Data) Systems. Three specimens were presumed as Heterosiphonia crispella by cluster analyses on DNA barcodes assisted by morphological identification

which was the first record in the investigated area

implying that it might be a cryptic or invasive species in the coastal area of northwestern Yellow Sea. In the neighbor-joining trees of all three DNA markers

Heterosiphonia japonica converged with Dasya spp. and was distant from the other Heterosiphonia spp.

implying that H. japonica had affinities to the genus Dasya. The LSU and UPA markers amplified and sequenced easier than the COI marker across the Ceramiales species

but the COI had a higher ability to discriminate between species.

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references

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