Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass

Shengjie Li; Linqiang Han; Junjie Bai; Dongmei Ma; Yingchun Quan; Jiajia Fan; Peng Jiang; Lingyun Yu

doi:10.1007/s00343-015-4081-2

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Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass

Updated：2023-05-16

- Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass
- Journal of Oceanology and Limnology Vol. 33, Issue 2, Pages: 328-338(2015)
- Affiliations：
  
  Pearl River Fisheries Research Institute Chinese Academy of Fisheries Sciences Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation China Ministry of Agriculture
- Author bio：
- Funds：
- DOI：10.1007/s00343-015-4081-2
  CLC：
- Online First：06 November 2014，
  
  Published：2015
- Accepted：
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Li, S., Han, L., Bai, J. et al. Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass., Chin. J. Ocean. Limnol. 33, 328–338 (2015). https://doi.org/10.1007/s00343-015-4081-2
DOI：

Li, S., Han, L., Bai, J. et al. Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass., Chin. J. Ocean. Limnol. 33, 328–338 (2015). https://doi.org/10.1007/s00343-015-4081-2 DOI：

摘要

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) has a wide range of biological functions. We cloned the full-length cDNAs encoding PACAP and PACAP-related peptide (PRP) from the brain of largemouth bass (Micropterus salmoides) and used real-time quantitative PCR to detect PRP-PACAP mRNA expression. The PRP-PACAP cDNA has two variants expressed via alternative splicing: a long form

which encodes both PRP and PACAP

and a short form

which encodes only PACAP. Sequence analysis results are consistent with a higher conservation of PACAP than PRP peptide sequences. The expression of PACAP-long and PACAP-short transcripts was highest in the forebrain

followed by the medulla

midbrain

pituitary

stomach

cerebellum

intestine

and kidney; however

these transcripts were either absent or were weakly expressed in the muscle

spleen

gill

heart

fatty tissue

and liver. The level of PACAP-short transcript expression was significantly higher than expression of the long transcript in the forebrain

cerebella

pituitary and intestine

but lower than that of the long transcript in the stomach. PACAP-long and PACAP-short transcripts were first detected at the blastula stage of embryogenesis

and the level of expression increased markedly between the muscular contraction stage and 3 d post hatch (dph). The expression of PACAP-long and PACAP-short transcripts decreased significantly in the brain following 4 d fasting compared with the control diet group. The down-regulation effect was enhanced as fasting continued. Conversely

expression levels increased significantly after 3 d of re-feeding. Our results suggest that PRP-PACAP acts as an important factor in appetite regulation in largemouth bass.

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references

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