Cloning and expression of follistatin gene in half-smooth tongue sole Cynoglossus semilaevis during the reproduction cycle

Haishen Wen; Yufeng Si; Yuanqing Zhang; Feng He; Jifang Li

doi:10.1007/s00343-015-4051-8

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Cloning and expression of follistatin gene in half-smooth tongue sole Cynoglossus semilaevis during the reproduction cycle

Updated：2023-05-16

- Cloning and expression of follistatin gene in half-smooth tongue sole Cynoglossus semilaevis during the reproduction cycle
- Journal of Oceanology and Limnology Vol. 33, Issue 2, Pages: 299-308(2015)
- Affiliations：
  
  1. College of Fisheries Ocean University of China
  2. College of Animal Science and Technology Inner Mongolia Nationality University
- Author bio：
- Funds：
- DOI：10.1007/s00343-015-4051-8
  CLC：
- Online First：21 October 2014，
  
  Published：2015
- Accepted：
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Wen, H., Si, Y., Zhang, Y. et al. Cloning and expression of follistatin gene in half-smooth tongue sole Cynoglossus semilaevis during the reproduction cycle., Chin. J. Ocean. Limnol. 33, 299–308 (2015). https://doi.org/10.1007/s00343-015-4051-8
DOI：

Wen, H., Si, Y., Zhang, Y. et al. Cloning and expression of follistatin gene in half-smooth tongue sole Cynoglossus semilaevis during the reproduction cycle., Chin. J. Ocean. Limnol. 33, 299–308 (2015). https://doi.org/10.1007/s00343-015-4051-8 DOI：

摘要

Abstract

Follistatin (FST) is a monomeric glycoprotein highly enriched in cysteines and belongs to TGF-β superfamily. FST can suppress the secretion of follicle-stimulating hormone and plays a vital role in the reproduction of vertebrates. We used rapid amplification of cDNA ends technology to clone the FST gene of half-smooth tongue sole

Cynoglossus semilaevis. We characterized its phylogenetic context and expression patterns to elucidate its function in the breeding season. The full-length sequence of FST is 1 455 bp and encodes a protein of 321 amino acids. We investigated the expression pattern of FST in C. semilaevis at different stages of reproduction using reverse transcription-polymerase chain reaction (RTPCR). FST mRNA was expressed in all 13 tissues analyzed

and was expressed at high levels in gonad and at slightly lower levels in gill and brain. During the reproductive cycle of C. semilaevis

the transcript level of FST was the highest in the perinucleolus stage

decreased in the primary yolk stage

slightly increased in the tertiary yolk stage

and then reduced to a minimal level in the atretic follicles stage of the ovary. We concluded that FST suppressed follicle-stimulating hormone

which stimulated oocyte development. However

no significant variation was observed across all stages of testis development

although the expression level in the spermatogenesis stage was relatively low

which may result from the regulation of FST by aromatase.

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references

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