Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding

Chunyu Xue; Bingwen Xi; Mingchun Ren; Jingjing Dong; Jun Xie; Pao Xu

doi:10.1007/s00343-015-4047-4

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Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding

Updated：2023-05-16

- Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding
- Journal of Oceanology and Limnology Vol. 33, Issue 2, Pages: 291-298(2015)
- Affiliations：
  
  1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Ministry of Agriculture Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences
  2. Wuxi Fishery College Nanjing Agricultural University
  3. Hubei Collaborative Innovation Center for Freshwater Aquaculture
- Author bio：
- Funds：
- DOI：10.1007/s00343-015-4047-4
  CLC：
- Online First：27 October 2014，
  
  Published：2015
- Accepted：
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Xue, C., Xi, B., Ren, M. et al. Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding., Chin. J. Ocean. Limnol. 33, 291–298 (2015). https://doi.org/10.1007/s00343-015-4047-4
DOI：

Xue, C., Xi, B., Ren, M. et al. Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding., Chin. J. Ocean. Limnol. 33, 291–298 (2015). https://doi.org/10.1007/s00343-015-4047-4 DOI：

摘要

Abstract

Mucins are important components of mucus

which form a natural

physical

biochemical and semipermeable mucosal layer on the epidermis of fish gills

skin

and the gastrointestinal tract. As the first step towards characterizing the function of Muc2

we cloned a partial Megalobrama amblycephala Muc2 cDNA of 2 175 bp

and analyzed its tissue-specific expression pattern by quantitative real-time PCR (qPCR). The obtained sequence comprised 41 bp 5′-untranslated region (5′-UTR)

2 134 bp open reading frame encoding a protein of 711 amino acids. BLAST searching and phylogenetic analysis showed that the predicted protein contained several common secreted mucin-module domains (VWD-C8-TIL-VWD-C8) and had high homology with mucins from other vertebrates. Among four candidate reference genes (β-Actin

RPI13α

RPII

18S) for the qPCR

RPII was chosen as an appropriate reference gene because of its lowest variation in different tissues. M. amblycephala Muc2 was mainly expressed in the intestine

in the order (highest to lowest) middle-intestine > fore-intestine > hind-intestine. Muc2 was expressed relatively poorly in other organs (brain

liver

kidney

spleen

skin and gill). Furthermore

after 20-days of starvation

M. amblycephala Muc2 expressions after refeeding for 0 h

3 h

16 h

3 d

and 10 d were significantly decreased in the three intestinal segments (P<0.05) at 16 h

and were then upregulated to near the initial level at 10 d.

关键词

Keywords

references

Abolfathi M, Hajimoradloo A, Ghorbani R, Zamani A. 2012. Effect of starvation and refeeding on digestive enzyme activities in juvenile roach, Rutilus rutilus caspicus. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 161(2): 166–173.

Bergstrom K S B, Kissoon-Singh V, Gibson D L, Ma C, Montero M, Sham H P, Ryz N, Huang T, Velcich A, Finlay B B, Chadee K, Vallance B A. 2010. Muc2 protects against lethal infectious colitis by disassociating pathogenic and commensal bacteria from the colonic mucosa. PloS Pathogens, 6(5): e1000902.

Corfield A P, Carroll D, Myerscough N, Probert C S. 2001. Mucins in the gastrointestinal tract in health and disease. Frontiers in Bioscience, 6(10): D1321–1357.

Esteban M A. 2012. An overview of the immunological defenses in fish skin. ISRN Immunology, http://dx.doi.org/10.5402/2012/853470.

Hansson G C. 2012. Role of mucus layers in gut infection and inflammation. Current Opinion in Microbiology, 15(1): 57–62, http://dx.doi.org/10.1016/j.mib.2011.11.002.

Hofer R. 1979. The adaptation of digestive enzymes to temperature, season and diet in roach, Rutilus rutilus and rudd Scardinius erythrophthalmus L. 1. Amylase. Journal of Fish Biology, 14(6): 565–572.

Jorgensen S M, Kleveland E J, Grimholt U, Gjoen T. 2006. Validation of reference genes for real-time polymerase chain reaction studies in Atlantic salmon. Marine Biotechnology, 8(4): 398–408, http://dx.doi.org/10.1007/s10126-005-5164-4.

Lang T, Hansson G C, Samuelsson T. 2007. Gel-forming mucins appeared early in metazoan evolution. Proceeding of the National Academy of Science, 104(41): 16 209–16 214, http://dx.doi.org/10.1073/pnas.0705984104.

Li C, Zhang Y, Wang R J, Lu J G, Nandi S, Mohanty S, Terhune J, Liu Z J, Peatman E. 2012. RNA-seq analysis of mucosal immune responses reveals signatures of intestinal barrier disruption and pathogen entry following Edwardsiella ictaluri infection in channel catfish, Ictalurus punctatus. Fish & Shellfish Immun ology, 32(5): 816–827. http://dx.doi.org/10.1016/j.fsi.2012.02.004.

Mayer L. 2003. Mucosal immunity. Pediatrics, 111(6): 1 595–1 600.

Ming J H, Xie J, Liu B, He Y J, Zhou Q L, Pan L K, Yu J H, Xu P. 2009. Cloning, sequence analysis of HSP70 cDNA and effects of heat stress on its mRNA expression in Megalobrama amblycephala. Journal of Fishery Sciences of China, 16(5): 635–648. (in Chinese with English abstract)

Ministry of Agriculture of the People’s Republic of China. 2012. China Fishery Statistical Yearbook. Chinese Agricultural Press, Beijing. p.28. (in Chinese)

Neuhaus H, Van der Marel M, Caspari N, Meyer W, Enss M L, Steinhagen D. 2007. Biochemical and histochemical effects of perorally applied endotoxin on intestinal mucin glycoproteins of the common carp Cyprinus carpio. Diseases of Aquatic Organisms, 77(1): 17–27.

Pérez-Sánchez J, Estensoro I, Redondo M J, Calduch-Giner J A, Kaushik S, Sitjà-Bobadilla A. 2013. Mucins as diagnostic and prognostic biomarkers in a fish-parasite model: transcriptional and functional analysis. PloS One, 8(6): e65457.

Roussel P, Delmotte P. 2004. The diversity of epithelial secreted mucins. Current Organic Chemistry, 8(5): 413–437.

Sahlmann C, Sutherland B J G, Kortner T M, Koop B F, Krogdahi A, Bakke A M. 2013. Early response of gene expression in the distal intestine of Atlantic salmon (Salmo salar L.) during the development of soybean meal induced enteritis. Fish & Shellfish Immunology, 34(2): 599–609, http://dx.doi.org/10.1016/j.fsi.2012.11.031.

Tang R, Dodd A, Lai D, Mcnabb W C, Love D R. 2007. Validation of Zebrafish (Danio rerio) reference genes for quantitative real-time RT-PCR normalization. Acta Biochimica et Biophysica Sinica, 39(5): 384–390.

Tang Y K, Yu J H, Xu P, Li J L, Li H X, Ren H T. 2012. Identification of housekeeping genes suitable for gene expression analysis in Jian carp (Cyprinus carpio var. jian). Fish & Shellfish Immunology, 33(4): 775–779, http://dx.doi.org/10.1016/j.fsi.2012.06.027.

Van der Marel M, Adamek M, Gonzalez S F, Frost P, Rombout J H W M, Wiegertjes G F, Savelkoul H F J, Steinhagen D. 2012. Molecular cloning and expression of two β-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after β-glucan feeding. Fish & Shellfish Immunology, 32(3): 494–501, http://dx.doi.org/10.1016/j.fsi.2011.12.008.

Van der Marel M, Caspari N, Neuhaus H, Meyer W, Enss M L, Steinhagen D. 2010. Change in skin mucus of common carp, Cyprinus carpio L., after exposure to water with a high bacterial load. Journal of Fish Diseases, 33(5): 431–439, http://dx.doi.org/10.1111/j.1365-2761.2010.01140.x.

Van Klinken B J W, Einerhand A W C, Duits L A, Makkink M K, Tytgat K M A J, Renes I B, Verburg M, Büller H A, Dekker J. 1999. Gastrointestinal expression and partial cDNA cloning of murine Muc2. American Journal of Physiology-Gastrointestinal and Liver Physiology, 276(1): G115–G124.

Wang T, Hung C, Randall D J. 2006. The comparative physiology of food deprivation from feast to famine. Annual Review of Physiology, 68: 223–251, http://dx.doi.org/10.1146/annurev.physiol.68.040104.105739.

Zeng L Q, Li F J, Li X M, Cao Z D, Fu S J, Zhang Y G. 2012. The effects of starvation on digestive tract function and structure in juvenile southern catfish (Silurus meridionalis Chen). Comparative Biochemistry and Physiology, Part A, 162: 200–211, http://dx.doi.org/10.1016/j.cbpa.2012.02.022.

Zhao Y H, Gul Y, Li S, Wang W M. 2011. Cloning, identification and accurate normalization expression analysis of PPARα gene by GeNorm in Megalobrama amblycephala. Fish & Shellfish Immunology, 31: 462–468, http://dx.doi.org/10.1016/j.fsi.2011.06.024.

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