A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance

Jingwen Liu; Yang Yu; Fuhua Li; Xiaojun Zhang; Jianhai Xiang

doi:10.1007/s00343-015-4010-4

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A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance

Updated：2023-05-16

- A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance
- Journal of Oceanology and Limnology Vol. 32, Issue 6, Pages: 1232-1247(2014)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. National & Local Joint Engineering Laboratory of Ecological Mariculture Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-015-4010-4
  CLC：
- Online First：07 August 2014，
  
  Published：2014
- Accepted：
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Liu, J., Yu, Y., Li, F. et al. A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance., Chin. J. Ocean. Limnol. 32, 1232–1247 (2014). https://doi.org/10.1007/s00343-015-4010-4
DOI：

Liu, J., Yu, Y., Li, F. et al. A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance., Chin. J. Ocean. Limnol. 32, 1232–1247 (2014). https://doi.org/10.1007/s00343-015-4010-4 DOI：

摘要

Abstract

Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF

designated nLvALF2

with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs

the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus (WSSV) infection

suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2

of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G

g.2466 T>C

and g.2529 G>A were significantly associated with the resistance to WSSV (P<0.05). These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei.

关键词

Keywords

references

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