Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg)

Yiyan Wang; Hushan Sun; Yanjie Wang; Dongchun Yan; Lei Wang

doi:10.1007/s00343-015-3297-5

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Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg)

Updated：2023-05-16

- Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg)
- Journal of Oceanology and Limnology Vol. 33, Issue 2, Pages: 339-346(2015)
- Affiliations：
  
  College of Life Science Ludong University
- Author bio：
- Funds：
- DOI：10.1007/s00343-015-3297-5
  CLC：
- Online First：06 November 2014，
  
  Published：2015
- Accepted：
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Wang, Y., Sun, H., Wang, Y. et al. Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg)., Chin. J. Ocean. Limnol. 33, 339–346 (2015). https://doi.org/10.1007/s00343-015-3297-5
DOI：

Wang, Y., Sun, H., Wang, Y. et al. Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg)., Chin. J. Ocean. Limnol. 33, 339–346 (2015). https://doi.org/10.1007/s00343-015-3297-5 DOI：

摘要

Abstract

In this study

a cytochemical method and transmission electron microscopy was used to examine acid phosphatase activities of yolk granules throughout the early developmental stages of the Pacific oyster Crassostrea gigas. This study aimed to investigate the dynamic change of yolk granule acid phosphatase

and the mechanisms underlying its involvement in yolk degradation during the early developmental stages of molluscs. Three types of yolk granules (YGI

YGII

and YGIII) that differed in electron density and acid phosphatase reaction were identified in early cleavage

morula

blastula

gastrula

trochophore

and veliger stages. The morphological heterogeneities of the yolk granules were related to acid phosphatase activity and degrees of yolk degradation

indicating the association of acid phosphatase with yolk degradation in embryos and larvae of molluscs. Fusion of yolk granules was observed during embryogenesis and larval development of C. gigas. The fusion of YGI (free of acid phosphatase reaction) with YGII (rich in acid phosphatase reaction) could be the way by which yolk degradation is triggered.

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references

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