Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas

Gang Liu; Pin Huan; Baozhong Liu

doi:10.1007/s00343-014-3360-7

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Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas

Updated：2023-05-16

- Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas
- Journal of Oceanology and Limnology Vol. 32, Issue 6, Pages: 1224-1231(2014)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3360-7
  CLC：
- Online First：24 May 2014，
  
  Published：2014
- Accepted：
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Liu, G., Huan, P. & Liu, B. Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas, ., Chin. J. Ocean. Limnol. 32, 1224–1231 (2014). https://doi.org/10.1007/s00343-014-3360-7
DOI：

Liu, G., Huan, P. & Liu, B. Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas, ., Chin. J. Ocean. Limnol. 32, 1224–1231 (2014). https://doi.org/10.1007/s00343-014-3360-7 DOI：

摘要

Abstract

Increasing evidence indicates that transforming growth factor β (TGF-β) signaling pathways play many important roles in the early development of mollusks. However

limited information is known concerning their detailed mechanisms. Here

we describe the identification

cloning and characterization of two Smad genes

the key components of TGF-β signaling pathways

from the Pacific oyster Crassostrea gigas. Sequence analysis of the two genes

designated as cgi-smad1/5/8 and cgi-smad4

revealed conserved functional characteristics. The two genes were widely expressed in embryos and larvae

suggesting multiple roles in the early development of C. gigas. The mRNA of the two genes aggregated in the D quadrant and cgi-smad4 was highly expressed on the dorsal side of the gastrula

indicating that TGF-β signaling pathways may be involved in dorsoventral patterning in C. gigas. Furthermore

high expression levels of the two genes in the shell fields of embryos at different stages suggested important roles for TGF-β signaling pathways in particular phases of shell development

including the formation of the initial shell field and the biomineralization of larval shells. The results of this study provide fundamental support for elucidating how TGF-β signaling pathways participate in the early development of bivalve mollusks

and suggest that further work is warranted to this end.

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