White spot syndrome virus inactivation study by using gamma irradiation

Marzieh Heidareh; Farahnaz Motamedi Sedeh; Mehdi Soltani; Saeed Rajabifar; Mohammad Afsharnasab; Aghil Dashtiannasab

doi:10.1007/s00343-014-3333-x

Your Location：

Home >

Browse articles >

White spot syndrome virus inactivation study by using gamma irradiation

Updated：2023-05-16

- White spot syndrome virus inactivation study by using gamma irradiation
- Journal of Oceanology and Limnology Vol. 32, Issue 5, Pages: 1024-1028(2014)
- Affiliations：
  
  1. Nuclear Science and Technology Research Institute
  2. Veterinary Faculty University of Tehran
  3. Iran Fisheries Research Institute
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3333-x
  CLC：
- Online First：02 July 2014，
  
  Published：2014
- Accepted：
Scan QR Code
Heidareh, M., Sedeh, F.M., Soltani, M. et al. White spot syndrome virus inactivation study by using gamma irradiation., Chin. J. Ocean. Limnol. 32, 1024–1028 (2014). https://doi.org/10.1007/s00343-014-3333-x
DOI：

Heidareh, M., Sedeh, F.M., Soltani, M. et al. White spot syndrome virus inactivation study by using gamma irradiation., Chin. J. Ocean. Limnol. 32, 1024–1028 (2014). https://doi.org/10.1007/s00343-014-3333-x DOI：

摘要

Abstract

The present study was conducted to investigate the effect of gamma irradiation on white spot syndrome virus (WSSV). White spot syndrome virus is a pathogen of major economic importance in cultured penaeid shrimp industries. White spot disease can cause mortalities reaching 100% within 3–10 days of gross signs appearing. During the period of culture

immunostimulant agents and vaccines may provide potential methods to protect shrimps from opportunistic and pathogenic microrganisms. In this study

firstly

WSSV was isolated from infected shrimp and then multiplied in crayfish. WSSV was purified from the infected crayfish haemolymph by sucrose gradient and confirmed by transmission electron microscopy. In vivo virus titration was performed in shrimp

Penaeus semisulcatus. The LD50 of live virus stock was calculated 10 5.4/mL. Shrimp post-larvae (1–2 g) were treated with gamma-irradiated (different doses) WSSV (100 to 10−4 dilutions) for a period of 10 days. The dose/survival curve for irradiated and un-irradiated WSSV was drawn; the optimum dose range for inactivation of WSSV and unaltered antigenicity was obtained 14–15 kGy. This preliminary information suggests that shrimp appear to benefit from treatment with gammairradiated WSSV especially at 14–15 KGy.

关键词

Keywords

references

Adams A. 1991. Response of penaeid shrimp to exposure to Vibrio species. Fish Shellfish Immunol., 1: 59–70.

Brown F. 2002. Inactivation of viruses by aziridines. Vaccine, 20: 322–327.

Cavalli L S, Marins L F, Netto S, Abreu P C. 2008. Evaluation of White Spot Syndrome Virus (WSSV) in wild shrimp after a major outbreak in shrimp farms at Laguna, southern Brazil. Atlântica Rio Grande, 30(1): 45–52.

Chang P S, Lo C F, Wang Y C, Kou G H. 1996. Identification of white spot syndrome associated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situ hybridization. Dis. Aquat. Organ., 27: 131–139.

Du H, Xu Z, Wu X, Li W, Dai W. 2006. Increased resistance to white spot syndrome virus in Procambarus clarkii by injection of envelope protein VP28 expressed using recombinant baculovirus. Aquaculture, 260: 39–43.

Escobedo-Bonilla C M, Wille M, Alday Sanz V, Sorgeloos Pensaert M B, Nauwynck J H. 2005. In vivo titration of white spot syndrome virus (WSSV) in specific pathogenfree Litopenaeus vannamei by intramuscular and oral routes. Dis. Aquat. Organ., 66: 163–170.

Ginoza W. 1968. Inactivation viruses by ionizing radiation and heat. In: Maramorosch K, Koprowski H eds. Methods in Virology, Vol.4. Academic Press, London, p.139–209.

Heidarieh M, Afsharnasab M, Soltani M, Dashtyannasab A, Rajabifar S, Sheikhzadeh N, Tamimi A H. 2010. Effects of Ergosan and Vibromax to prevent vibriosis and WSSV in Litopenaeus vannamei. J. Fish. Aquat. Sci., 5: 120–125.

Heidarieh M, Hedayatirad Maryam, MirvaghefiA R, Diallo A, Mousavi Sh, Sheikhzadeh N, Shahbazfar A A. 2014. Effect of gamma-irradiation on inactivation of Ichthyophthirius multifiliis trophonts and its efficacy on host response in experimentally immunized rainbow trout (Oncorhynchus mykiss). Turk. J. Vet. Anim. Sci., http://dx.doi.org/10.3906/vet-1312-78.

Heidarieh M, Soltani M, Motamedi Sedeh F, Sheikhzadeh N. 2013. Low water temperature retards white spot syndrome virus replication in Astacus leptodactylus Crayfish. Acta Sci. Vet., 41: 1 135.

Huang C H, Zhang L R, Zhang J H, Xiao L C, Wu Q J, Chen D H, Li J K K. 2001. Purification and characterization of white spot syndrome virus (WSSV) produced in an alternate host: crayfish, Cambarus clarkii. Virus Res., 76: 115–125.

Itami T, Takahashi Y, Nakamura Y. 1989. Efficacy of vaccination against vibriosis in cultured kuruma prawns Penaeus japonicus. J. Aquat. Anim. Health, 1: 234–242.

Karber G. 2002. Foot and mouth disease, Karber Formula for calculation of virus/antibody titres. OIE A Manual, Overview.

Lombardo J H, Smolko E E. 1990. A Biotechnological project with a gamma radiation source of 100,000 Ci. Radiat. Phys. Chem., 35(4–6): 585–589.

Motamedi Sedeh F, Afsharnasab M, Heidarieh M, Shafaee S K, Rajabifar S, Dashtiannasab A, Razavi M H. 2012. Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus. Iran. J. Fish. Sci., 11(1): 145–155.

Motamedi Sedeh F, Khorasani A, Shafaee K, Fatolahi H, Arbabi K. 2008. Preparation of FMD type A87/IRN inactivated vaccine by gamma irradiation and the immune response on guinea pig. Indian J. Microbiol., 48(3): 326–330.

Motamedi Sedeh F, Soleimanjahi H, Jalilian A, Mahravani H. 2011. Inactivation of nano-FMDV type O/IRN/1/2007 particles infectivity using gamma irradiation. Indian J. Sci. Technol., 4(12): 1 654–1 656.

Namikoshi A, Wu J L, Yamashita T, Nishizawa T, Nishioka T, Arimoto M, Muroga K. 2004. Vaccination trials with Penaeus japonicus to induce resistance to white spot syndrome virus. Aquaculture, 229: 25–35.

Ning J F, Zhu W, Xu J P, Zheng C Y, Meng X L. 2009. Oral delivery of DNA vaccine encoding VP28 against white spot syndrome virus in crayfish by attenuated Salmonella typhimurium. Vaccine, 27: 1 127–1 135.

Pollard E. 1955. The action of ionizing radiation on viruses. Advan. Virus Res., 2: 109–151.

Poulos B T, Pantoja C R, Bradley-Dunlop D, Aguilar J, Lightner D V. 2001. Development and application of monoclonal antibodies for the detection of white spot syndrome virus of Penaeid shrimp. Dis. Aquat. Organ., 47: 13–23.

Rajesh Kumar S, Ishaq Ahamed V P, Sarathi M, Nazeer Basha A, Sahul Hameed A S. 2008. Immunological responses of Penaeus monodon to DNA vaccine and its efficacy to protect shrimp against white spot syndrome virus (WSSV). Fish Shellfish Immunol., 24(4): 467–478.

Rowley A F, Powell A. 2007. Invertebrate immune systemsspecific, quasi-specific, or nonspecific? J. Immunol., 179: 7 209–7 214.

Smolko E E, Lombardo J H. 2005. Virus inactivation studies using ion beams, electron and gamma irradiation. Nucl. Instr. Meth. Phys. B, 236: 249–253.

Syaifudin M, Tetriana D, Darlina Nurhayati S. 2011. The feasibility of gamma irradiation for developing malaria vaccine. Atom Indones., 37(3): 91–101.

Traifalgar R F M, Corre V L, Serrano A E. 2013. Efficacy of dietary immunostimulants to enhance the immunological responses and Vibriosis resistance of juvenile Penaeus monodon. J. Fish. Aquat. Sci., 8: 340–354.

Tsai M F, Yu H T, Tzeng H F, Leu J H, Chou C M, Huang C J, Wang C H, Lin J Y, Kou G H, Lo C F. 2000. Identification and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virolog, 277: 100–110.

van Hulten M C W, Tsai M F, Schipper C A, Lo C F, Kou G H, Valk J M. 2000. Analysis of a genomic segment of white spot syndrome virus of shrimp containing ribonucleotide reductase genes and repeat regions. J. Gen. Virol., 81: 307–316.

van Hulten M C W, Witteveldt J, Snippe M, Valk J M. 2001. White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp. Virology, 285: 228–233.

Wang Y T, Liu W, Seah J N, Lam C S, Xiang J H, Korzh V, Kwang J. 2002. White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis. Dis. Aquat. Organ., 52: 249–259.

Witteveldt J, Cifuentes C C, Valk J M, Van Hulten M C W. 2004a. Protection of Penaeus monodo n against white spot syndrom virus by oral vaccination. J. Virol., 78(4): 2 057–2 061.

Witteveldt J, Valk J M, van Hulten MC W. 2004b. Protection of Penaeus monodon against white spot syndrome virus using a WSSV subunit vaccine. Fish Shellfish Immunol., 16: 571–579.

Wu J L, Nishioka T, Nishizaw T, Muroga K. 2002. A timecourse study on the resistance of Penaeus japonicus induced by artificial infection with white spot syndrome virus. Fish Shellfish Immunol., 13(5): 391–403.

Zhu F, Du H, Miao Z G, Quan H Z, Xu Z R. 2009. Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV. Fish Shellfish Immunol., 26: 685–690.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

AVibrio anguillarum strain associated with skin ulcer on cultured flounder,Paralichthys olivaceus

Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

Development and application of antibody microarray for white spot syndrome virus detection in shrimp

Influence of Structural Parameters of Diversion Cone on Smooth Effect of Gas Ejection Bottom Pressure Impact

Multivariable Constrained Model Predictive Control ofAero-Engine Based on Augmented Predictive Model

Related Author

Mo Zhao-lan

Tan Xun-gang

Xu Yong-li

Zhang Pei-jun

Zhaolan Mo

Dongsheng Guo

Yunxiang Mao

Xuhong Ye

Related Institution

Experimental Marine Biology Laboratory Institute of Oceanology Chinese Academy of Sciences

Institute of Oceanology Chinese Academy of Sciences

Graduate University of Chinese Academy of Sciences

Ocean University of China

Laboratory of Pathology and Immunology of Aquatic Animals LMMEC Ocean University of China

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰