Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite

Zuoyong Luo; Baojie Wang; Mei Liu; Keyong Jiang; Mingxing Liu; Lei Wang

doi:10.1007/s00343-014-3310-4

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Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite

Updated：2023-05-16

- Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite
- Journal of Oceanology and Limnology Vol. 32, Issue 4, Pages: 749-763(2014)
- Affiliations：
  
  1. National & Local Joint Engineering Laboratory for Ecological Mariculture Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3310-4
  CLC：
- Online First：11 July 2014，
  
  Published：2014
- Accepted：
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Luo, Z., Wang, B., Liu, M. et al. Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite., Chin. J. Ocean. Limnol. 32, 749–763 (2014). https://doi.org/10.1007/s00343-014-3310-4
DOI：

Luo, Z., Wang, B., Liu, M. et al. Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite., Chin. J. Ocean. Limnol. 32, 749–763 (2014). https://doi.org/10.1007/s00343-014-3310-4 DOI：

摘要

Abstract

Different amounts of vitamin C were added to diets fed to juveniles (2.5 ± 0.15 g) of sea cucumber Apostichopus japonic u s (Selenka) in an attempt to reduce the stress response of specimens exposed to nitrite stress. A commercial feed was used as the control diet and three experimental diets were made by supplementing 1 000

1 500

or 2 000 mg vitamin C/kg diet to control diet separately in a 45-day experiment. Sea cucumbers were exposed to three different levels (0.5

1.0

and 1.5 mg/L) of nitrite stress for 4

and 12 h at four time intervals (0

and 45 d). Growth of the animals was recorded during the experiment. Reactive oxygen species (ROS) (i.e. hydroxyl free radical (-OH)

malondialdehyde (MDA) and total antioxidant capacity (T-AOC)) and antioxidant enzyme activities (i.e.

superoxide dismutase (SOD) and catalase (CAT)) were measured. Response surface methodology (RSM) was used to analyze the effect of multiple factors on ROS indices and enzyme activities. Weight gain (WG) and special growth rate (SGR) of vitamin C supplementation groups were significantly higher than those of control group (P < 0.05). The levels of -OH and MDA increased under exposure time extending and nitrite concentration increasing

whereas T-AOC level decreased. SOD and CAT activities increased at 4 h and 8 h and decreased at 12 h. During the days in which the animal consumed experimental diets

the levels of -OH and MDA decreased and that of T-AOC increased. This result suggests that diets containing vitamin C could reduce the nitrite stress response in the animals and increase their antioxidant capacity. The multifactor regression equation of growth performance

ROS indices

and duration of feeding results suggest that vitamin C supplementation of 1 400–2 000 mg/kg diet for 29–35 days could reduce effectively the effects of nitrite exposure.

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references

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