Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei

Lingwei Ruan; Rongdiao Liu; Xun Xu; Hong Shi

doi:10.1007/s00343-014-3295-z

Your Location：

Home >

Browse articles >

Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei

Updated：2023-05-16

- Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei
- Journal of Oceanology and Limnology Vol. 32, Issue 4, Pages: 792-798(2014)
- Affiliations：
  
  Key Laboratory of Marine Biogenetic Resources Third Institute of Oceanography State Oceanic Administration (SOA)
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3295-z
  CLC：
- Online First：11 July 2014，
  
  Published：2014
- Accepted：
Scan QR Code
Ruan, L., Liu, R., Xu, X. et al. Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei, ., Chin. J. Ocean. Limnol. 32, 792–798 (2014). https://doi.org/10.1007/s00343-014-3295-z
DOI：

Ruan, L., Liu, R., Xu, X. et al. Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei, ., Chin. J. Ocean. Limnol. 32, 792–798 (2014). https://doi.org/10.1007/s00343-014-3295-z DOI：

摘要

Abstract

The phosphatidylinositol 3-kinase (PI3K)-AKT pathway is involved in various cellular functions

including anti-apoptosis

protein synthesis

glucose metabolism and cell cycling. However

the role of the PI3K-AKT pathway in crustaceans remains unclear. In the present study

we cloned and characterized the AKT gene lvakt from Litopenaeus vannamei. The 511-residue LVAKT was highly conserved; contained a PH domain

a catalytic domain and a hydrophobic domain; and was highly expressed in the heart and gills of L. vannamei. We found

using Real-Time Quantitative PCR (Q-PCR) analysis

that lvakt was up-regulated during early white spot syndrome virus (WSSV) infection. Moreover

the PI3K-specific inhibitor

LY294002

reduced viral gene transcription

implying that the PI3K-AKT pathway might be hijacked by WSSV. Our results therefore suggest that LVAKT may play an important role in the shrimp immune response against WSSV.

关键词

Keywords

references

Bellacosa A, Testa J R, Staal S P, Tsichlis P N. 1991. A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region. Science, 254: 274–277.

Bottomley M J, Salim K, Panayotou G. 1998. Phospholipidbinding protein domains. Biochim. Biophys. Acta, 1436: 165–183.

Cantrell D A. 2001. Phosphoinositide 3-kinase signalling pathways. J. Cell Sci., 114: 1 439–1 445.

Chen W Y, Ho K C, Leu J H, Liu K F, Wang H C, Kou G H, Lo C F. 2008. WSSV infection activates STAT in shrimp. Dev. Comp. Immunol., 32: 1 142–1 150.

Cooray S. 2004. The pivotal role of phosphatidylinositol 3-kinase-Akt signal transduction in virus survival. J. Gen. Virol., 85: 1 065–1 076.

Dawson C W, Tramountanis G, Eliopoulos A G, Young L S. 2003. Epstein-Barr virus latent membrane protein 1 (LMP1) activates the phosphatidylinositol 3-kinase/Akt pathway to promote cell survival and induce actin filament remodeling. J. Biol. Chem., 278: 3 694–3 704.

Downward J. 1998. Mechanisms and consequences of activation of protein kinase B/Akt. Curr. Opin. Cell Biol., 10: 262–267.

Huang X D, Zhao L, Zhang H Q, Xu X P, Jia X T, Chen Y H, Wang P H, Weng S P, Yu X Q, Yin Z X, He J G. 2010. Shrimp NF-kappaB binds to the immediate-early gene ie1 promoter of white spot syndrome virus and upregulates its activity. Virology, 406: 176–180.

Ji W T, Liu H J. 2008. PI3K-Akt signaling and viral infection. Recent Pat. Biotechnol., 2: 218–226.

Liu W J, Chang Y S, Wang A H, Kou G H, Lo C F. 2007. White spot syndrome virus annexes a shrimp STAT to enhance expression of the immediate-early gene ie1. J. Virol., 81: 1 461–1 471.

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods, 25: 402–408.

Manning B D, Cantley L C. 2007. AKT/PKB signaling: navigating downstream. Cell, 129: 1 261–1 274.

Patel R K, Hardy R W. 2012. Role for the phosphatidylinositol 3-kinase-Akt-TOR pathway during sindbis virus replication in arthropods. J. Virol., 86: 3 595–3 604.

Saeed M F, Kolokoltsov A A, Freiberg A N, Holbrook M R, Davey R A. 2008. Phosphoinositide-3 kinase-Akt pathway controls cellular entry of Ebola virus. PLoS Pathog., 4: e1000141.

Saitou N, Nei M. 1987. The Neighbor-Joining Method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4: 406–425.

Sarbassov D D, Guertin D A, Ali S M, Sabatini D M. 2005. Phosphorylation and regulation of Akt/PKB by the rictormTOR complex. Science, 307: 1 098–1 101.

Shin Y K, Liu Q, Tikoo S K, Babiuk L A, Zhou Y. 2007. Effect of the phosphatidylinositol 3-kinase/Akt pathway on influenza A virus propagation. J. Gen. Virol., 88: 942–950.

Soares J A, Leite F G, Andrade L G, Torres A A, De Sousa L P, Barcelos L S, Teixeira M M, Ferreira P C, Kroon E G, Souto-Padron T, Bonjardim C A. 2009. Activation of the PI3K/Akt pathway early during vaccinia and cowpox virus infections is required for both host survival and viral replication. J. Virol., 83: 6 883–6 899.

Song K K, Li D F, Zhang M C, Yang H J, Ruan L W, Xu X. 2010. Cloning and characterization of three novel WSSV recognizing lectins from shrimp Marsupenaeus japonicus. Fish Shellfish Immunol., 28: 596–603.

Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24: 1 596–1 599.

Thompson J D, Higgins D G, Gibson T J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res., 22: 4 673–4 680.

Vogt P K, Hart J R, Gymnopoulos M, Jiang H, Kang S, Bader A G, Zhao L, Denley A. 2010. Phosphatidylinositol 3-kinase: the oncoprotein. Curr. Top Microbiol. Immunol., 347: 79–104.

Wang P H, Gu Z H, Wan D H, Liu B D, Huang X D, Weng S P, Yu X Q, He J G. 2013. The shrimp IKK-NF-kappaB signaling pathway regulates antimicrobial peptide expression and may be subverted by white spot syndrome virus to facilitate viral gene expression. Cell Mol. Immunol., 10: 423–436.

Wang P H, Gu Z H, Wan D H, Zhang M Y, Weng S P, Yu X Q, He J G. 2011. The shrimp NF-kappaB pathway is activated by white spot syndrome virus (WSSV) 449 to facilitate the expression of WSSV069 (ie1), WSSV303 and WSSV371. PLoS One, 6: e24773.

Xiao W, Yang Y, Weng Q, Lin T, Yuan M, Yang K, Pang Y. 2009. The role of the PI3K-Akt signal transduction pathway in Autographa californica multiple nucleopolyhedrovirus infection of Spodoptera frugiperda cells. Virology, 391: 83–89.

Xie X, Li H, Xu L, Yang F. 2005. A simple and efficient method for purification of intact white spot syndrome virus (WSSV) viral particles. Virus Res., 108: 63–67.

Zhou Q, Qi Y P, Yang F. 2007. Application of spectrophotometry to evaluate the concentration of purified White Spot Syndrome Virus. J. Virol. Methods, 146: 288–292.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

Impact of Vibrio parahaemolyticus and white spot syndrome virus (WSSV) co-infection on survival of penaeid shrimp Litopenaeus vannamei

VP39A is a novel host cellular binding protein of white spot syndrome virus and interacts with Na⁺, K⁺-ATPase alpha subunit

EST-derived SNP discovery and selective pressure analysis in Pacific white shrimp (Litopenaeus vannamei)

Inhibitor effects on the anodic behavior of iron electrodes

Related Author

Xie Shijun

Li Fuhua

Zhang Xiaojun

Zhang Jiquan

Xiang Jianhai

Xiaojing Zhang

Xiaoling Song

Jie Huang

Related Institution

Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology

University of Chinese Academy of Sciences

Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences

Qingdao National Laboratory for Marine Science and Technology

Key Laboratory for Sustainable Development of Marine Fisheries Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Science

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰