Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

Yang Yu; Xiaojun Zhang; Jingwen Liu; Fuhua Li; Hao Huang; Yijun Li; Xiaolin Liu; Jianhai Xiang

doi:10.1007/s00343-014-3232-1

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Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

Updated：2023-05-16

- Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei
- Journal of Oceanology and Limnology Vol. 33, Issue 1, Pages: 1-10(2015)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Hainan Nanjiang Marine Biotechnique Company Limited
  4. Northwest Agriculture and Forestry University
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3232-1
  CLC：
- Online First：27 October 2014，
  
  Published：2015
- Accepted：
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Yu, Y., Zhang, X., Liu, J. et al. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei, ., Chin. J. Ocean. Limnol. 33, 1–10 (2015). https://doi.org/10.1007/s00343-014-3232-1
DOI：

Yu, Y., Zhang, X., Liu, J. et al. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei, ., Chin. J. Ocean. Limnol. 33, 1–10 (2015). https://doi.org/10.1007/s00343-014-3232-1 DOI：

摘要

Abstract

Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance. However

the identification of new varieties of shrimps from their phenotypic characters is difficult. This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences. The method was employed to identify a new selected variety

Kehai No. 1 (KH-1)

from three representative stocks (control group): Zhengda; Tongwei; and a stock collected from Fujian Province

which is now cultured in mainland China. By pooled genotyping of KH-1 and the control group

five microsatellites showing differences between KH-1 and the control group were screened out. Individual genotyping data confirmed the results from pooled genotyping. The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2. By sequencing the mitochondrial control regions of individuals from the KH-1 and control group

four haplotypes were observed in the KH-1 group

whereas 14 haplotypes were obtained in the control group. By combining the microsatellite assignment analysis with mitochondrial control region analysis

the average accuracy of identification of individuals in the KH-1 group and control group reached 89%. The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L. vannamei from other populations cultured in China.

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references

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