Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique

Wei Zhou; Hongye Ding; Zhenghong Sui; Zhongxia Wang; Jinguo Wang

doi:10.1007/s00343-014-3209-0

Your Location：

Home >

Browse articles >

Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique

Updated：2023-05-16

- Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique
- Journal of Oceanology and Limnology Vol. 32, Issue 3, Pages: 522-526(2014)
- Affiliations：
  
  Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3209-0
  CLC：
- Online First：04 May 2014，
  
  Published：2014
- Accepted：
Scan QR Code
Zhou, W., Ding, H., Sui, Z. et al. Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique., Chin. J. Ocean. Limnol. 32, 522–526 (2014). https://doi.org/10.1007/s00343-014-3209-0
DOI：

Zhou, W., Ding, H., Sui, Z. et al. Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique., Chin. J. Ocean. Limnol. 32, 522–526 (2014). https://doi.org/10.1007/s00343-014-3209-0 DOI：

摘要

Abstract

The red alga Gracilariopsis lemaneiformis (Bory) is an economically valuable macroalgae. As a means to identify the sex of immature Gracilariopsis lemaneiformis

the amplified fragment length polymorphism (AFLP) technique was used to search for possible sex- or phase-related markers in male gametophytes

female gametophytes

and tetrasporophytes

respectively. Seven AFLP selective amplification primers were used in this study. The primer combination E-TG/M-CCA detected a specific band linked to male gametophytes. The DNA fragment was recovered and a 402-bp fragment was sequenced. However

no DNA sequence match was found in public databases. Sequence characterized amplified region (SCAR) primers were designed from the sequence to test the repeatability of the relationship to the sex

using 69 male gametophytes

139 female gametophytes

and 47 tetrasporophytes. The test results demonstrate a good linkage and repeatability of the SCAR marker to sex. The SCAR primers developed in this study could reduce the time required for sex identification of Gracilariopsis lemaneiformis by four to six months. This can reduce both the time investment and number of specimens required in breeding experiments.

关键词

Keywords

references

Chen S L, Li J, Deng S P, Tian Y S, Wang Q Y, Zhuang Z M, Sha Z X, Xu J Y. 2007. Isolation of female-specific AFLP markers and molecular identification of genetic sex in half-smooth tongue sole (Cynoglossus semilaevis). Mar. Bio., 9: 273–280.

Huan L, He L W, Zhang B Y, Niu J F, Lin A P, Wang G C. 2013. AFLP and SCAR markers associated with the sex in Gracilaria lemaneiformis (Rhodophyta). J. Phycol., 49: 728–732.

Lapointe B E, Ryther J H. 1978. Some aspects of the growth and yield of Gracilaria tikvahiae in culture. Aquaculture, 15: 185–193.

Ma H Y, Chen S L, Yang J F, Ji X S, Chen S Q, Tian Y S, Bi J Z. 2010. Isolation of sex-specific AFLP markers in spotted halibut (Verasper variegatus). Environ. Biol. Fish., 88: 9–14.

McHugh D J. 1991. Worldwide distribution of commercial resources of seaweeds including Gelidium. Hydrobiologia, 221: 19–29.

Meng L, Xu D, Chen W Z, Zhang X C. 2009. Selection and characterization of a new strain of Gracilaria lemaneiformis. Peri. Ocean Univ. Chin., 39(Sup): 94–98. (in Chinese with English abstract)

Pflugmacher S, Steinberg C E W, Sandermann H. 1997. Activity of phase I and phase II detoxification enzymes in aquatic macrophytes. Angewandte Botanik, 71: 144–146.

Qiao L X, Liu H Y, Guo B T, Feng Y B, Weng M L, Dai J X, Duan D, Wang B. 2007. Molecular identification of 16 Porphyra lines using sequence-related amplified polymorphism markers. Aquat. Bot., 87: 203–208.

Ren X Y, Zhang X C. 2008. Identification of a putative tetrasporophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte). J. Ocean Univ. Chin., 7: 299–303.

Schmidt H, Ehrmann M, Vogela R F, Taniwakib M H, Niessena L. 2003. Molecular typing of Aspergillus ochraceus and construction of species specific SCAR Primers based on AFLP. Syst. Appl. Microbiol., 26: 138–146.

Sun J W, Jin D M, Zhou C J, Yang Q K, Weng M L, Duan D L, Xu P, Ma J H, Wang B. 2005. Identification of Porphyr a lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers. Plant Mol. Biol. Rep., 23: 251–262.

Sun X, Zhang X C, Mao Y X, Sui Z H, Qin S. 2006. Identification of phase and sex-related ISSR markers of red alga Gracilaria lemaneiformis. J. Ocean Univ. Chin., 5: 82–84.

Vos P, Hogers R, Bieeker M, Reijans M, Van der lee T, Hornes M, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nuc. Acids Res., 23: 4 407–4 414.

Wang C, Dong D, Wang G C, Zhang B Y, Peng G, Xu P, Tang X R. 2013. An improved PCR method for identification of Porphyra (Bangiales, Rhodophyta) directly using conchocelis based on RUBISCO intergenic spacer. Chin. J. Oceanol. Limnol., 27: 513–518.

Wang W J, Wang G C, Gao Z G, Lin X Z, Xu P. 2007b. Characterization of Gracilaria lemaneiformis Bory (Gracilariaceae, Rhodophyta) cultivars in China using the total soluble proteins and RAPD analysis. Bot. Mar., 50: 177–184.

Wang Y, Yu Z M, Song X X, Tang X X, Zhang S D. 2007a. Effects of macroalgae Ulva pertusa (Chlorophyta) and Gracilaria lemaneiformis (Rhodophyta) on growth of four species of bloom-forming dinoflagellates. Aquat. Bot., 86: 139–147.

Wang Z Y, Wang G C, Niu J F, Wang W J, Peng G. 2010. Optimization of conditions for tetraspore release and assessment of photosynthetic activities for different generation branches of Gracilaria leman eiformis Bory. Chin. J. Oceanol. Limnol., 28: 738–748.

Weng M L, Liu B, Jin D M, Yang Q K, Zhao G, Ma J H, Xu P, Duan D L, Wang B. 2005. Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers. J. Appl. Phycol., 17: 91–97.

Zhang X C, Qin S, Ma J H, Xu P. 2005. Genetics of marine red algae — Gracilaria. In: Lin Z Y ed. The Genetics of Marine Alga. China Agriculture Press, Beijing. p.236–283. (in Chinese)

Zhang X C, Van der Meer J P. 1988. A genetic study on Gracilaria sjoestedtii. Can. J. Bot., 66: 2 022–2 026.

Zhou Y, Yang H S, Hu H Y, Liu Y, Mao Y Z, Zhou H, Xu X L, Zhang F S. 2006. Bioremediation potential of the macroalga Gracilaria lemaneiformis (Rhodophyta) integrated into fed fish culture in coastal waters of north China. Aquaculture, 252: 264–276.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Sinosiphonia, a new red algal genus of the rhodomelaceae from north China

Biomass and carbon storage of Gracilariopsis lemaneiformis (Rhodophyta) in Zhanshan Bay, Qingdao, China

Wall Corrosion Morphology Prediction of Hall ThrustersUsing Semi-Empirical Method

Analysis Procedure and Numerical Simulation of Failure Probability of Turbine Disk Caused by Defects

Numerical Studies on Effects of V-Shaped Leading Edge on Incident Shock Wave Boundary Layer Interaction

Related Author

C. K. Tseng

Zheng Bailin

Wei Zhou

Zhenghong Sui

Jinguo Wang

Yiyi Hu

Kyoungho Kang

Junyeong Oh

Related Institution

Institute of Oceanology

Key Laboratory of Marine Genetics and Breeding Ministry of Education College of Marine Life Sciences Ocean University of China

Department of Aquaculture Chonnam National University

Aquaculture Technology Extension Station of Putian

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰