Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress

Zhixiang Liu; Hongli Cui; Zhengyi Liu; Yinchu Wang; Yulin Cui; Zhaopu Liu; Song Qin

doi:10.1007/s00343-014-3174-7

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Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress

Updated：2023-05-16

- Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress
- Journal of Oceanology and Limnology Vol. 32, Issue 2, Pages: 403-418(2014)
- Affiliations：
  
  1. College of Resources and Environmental Sciences Key Laboratory of Marine Biology Nanjing Agricultural University
  2. Yantai Institute of Coastal Zone Research Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3174-7
  CLC：
- Online First：06 March 2014，
  
  Published：2014
- Accepted：
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Liu, Z., Cui, H., Liu, Z. et al. Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress., Chin. J. Ocean. Limnol. 32, 403–418 (2014). https://doi.org/10.1007/s00343-014-3174-7
DOI：

Liu, Z., Cui, H., Liu, Z. et al. Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress., Chin. J. Ocean. Limnol. 32, 403–418 (2014). https://doi.org/10.1007/s00343-014-3174-7 DOI：

摘要

Abstract

In this study

cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays

899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes

353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10°C)

including genes for photosystem I

photosystem II

photosynthetic electron transport

and cytochrome b6/f complex. Moreover

desB

one of four genes that encode the fatty acid desaturases

was also induced by LT. However

the LT conditions to some degree enhanced the transcription of some genes. In addition

LT (10°C) may reduce cellular motility by regulating the transcription of spkA (sll1575)

a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT

including pathways involved in photosynthesis and repair.

关键词

Keywords

references

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