Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum

Lin Zhang; Jichang Han; Guanpin Yang; Baohua Zhu; Kehou Pan

doi:10.1007/s00343-014-3173-8

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Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum

Updated：2023-05-16

- Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum
- Journal of Oceanology and Limnology Vol. 32, Issue 2, Pages: 397-402(2014)
- Affiliations：
  
  1. Laboratory of Applied Microalgae Biology Key Laboratory of Mariculture of Ministry of Education of China Ocean University of China
  2. College of Marine Life Sciences Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3173-8
  CLC：
- Online First：06 March 2014，
  
  Published：2014
- Accepted：
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Zhang, L., Han, J., Yang, G. et al. Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum, ., Chin. J. Ocean. Limnol. 32, 397–402 (2014). https://doi.org/10.1007/s00343-014-3173-8
DOI：

Zhang, L., Han, J., Yang, G. et al. Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum, ., Chin. J. Ocean. Limnol. 32, 397–402 (2014). https://doi.org/10.1007/s00343-014-3173-8 DOI：

摘要

Abstract

Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently

it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids (mainly triacylglycerides

or TAGs) of microalgae

including P. tricornutum

but the mechanism is unclear. In this study

we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P. tricornutum. After being cultured under nitrogen-free conditions for 0

120

and 168 h

the TAG contents of P. tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR. The results show that the abundances of four target gene transcripts (LACS3

G3PDH2

G3PDH3

and G3PDH5) were positively correlated with TAG content

indicating that these genes may be involved in TAG synthesis in P. tricornutum. The findings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content of P. tricornutum.

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references

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