Assessment of parental contribution and effective population size from a 3×3 diallel cross of clam Meretrix meretrix

Ping Dai; Hongxia Wang; Baozhong Liu

doi:10.1007/s00343-014-3149-8

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Assessment of parental contribution and effective population size from a 3×3 diallel cross of clam Meretrix meretrix

Updated：2023-05-16

- Assessment of parental contribution and effective population size from a 3×3 diallel cross of clam Meretrix meretrix
- Journal of Oceanology and Limnology Vol. 32, Issue 2, Pages: 306-315(2014)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3149-8
  CLC：
- Online First：06 March 2014，
  
  Published：2014
- Accepted：
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Dai, P., Wang, H. & Liu, B. Assessment of parental contribution and effective population size from a 3×3 diallel cross of clam Meretrix meretrix, ., Chin. J. Ocean. Limnol. 32, 306–315 (2014). https://doi.org/10.1007/s00343-014-3149-8
DOI：

Dai, P., Wang, H. & Liu, B. Assessment of parental contribution and effective population size from a 3×3 diallel cross of clam Meretrix meretrix, ., Chin. J. Ocean. Limnol. 32, 306–315 (2014). https://doi.org/10.1007/s00343-014-3149-8 DOI：

摘要

Abstract

Unbalanced parental contribution and small effective population size (N e) are common issues during the artificial breeding of marine bivalves. The impact of hatchery-spawning practices on parental contribution

effective population size

the N e/N ratio

and genetic diversity are largely unknown. To address this

we conducted a parentage analysis on a complete 3×3 diallel cross of clam M eretrix meretrix using eight microsatellite markers. The genetic diversity of the parents was higher than that of their respective offspring in most crosses (8/9). Sires or dams from the same family contributed unequally to the pool of offspring from a particular cross

and the same parent clam exhibited large variation in parental contribution among different crosses. The variance in male contribution was higher than that of the female contribution in most crosses

suggesting that male contribution was more skewed than for females. The N e/N ratio for nine crosses ranged from 0.58 to 0.86. There was no linear relationship between the sex ratio and the N e/N ratio (P > 0.05). Moreover

a sex ratio closer to one-to-one does not necessarily mean a larger effective population size. A solution to small effective population size in commercial breeding programs is increasing broodstock numbers and attempting to maintain a balanced sex ratio.

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references

Aho T, Ronn J, Piironen J, Bjorklund M. 2006. Impacts of effective population size on genetic diversity in hatchery reared Brown trout (Salmo trutta L.) populations. Aquaculture, 253: 244–248.

Allendorf F W, Ryman N. 1987. Genetic management of hatchery stocks. In: Ryman N, Utter F eds. Population Genetics and Fishery Management. Washington Sea Grant Publications, University Washington Press, Seattle and London. p.141–159.

Appleyard S A, Ward R D. 2006. Genetic diversity and effective population size in mass selection lines of Pacific oyster (Crassostrea gigas). Aquaculture, 254: 148–159.

Bardon A, Vandeputte M, Dupont-Nive M, Chavanne H, Haffray P, Vergnet A, Chatain B. 2009. What is the heritable component of spinal deformities in the European sea bass (Dicentrarchus labrax)? Aquaculture, 294(3–4): 194–201.

Bekkevold D, Hansen M M, Loeschcke V. 2002. Male reproductive competition in spawning aggregations of cod (Gadus morhua L.). Mol. Ecol., 11: 91–102.

Bernatchez L, Duchesne P. 2000. Individual-based genotype analysis in studies of parentage and population assignment: how many loci, how many alleles? Can. J. Fish. Aquat. Sci., 57: 1–12.

Bijma P, Meuwissen T H E, Woolliams J A. 2002. Design of sustainable breeding programs in developed countries. Proceedings of the 7th Congress on Genetics Applied to Livestock Production, 33: 265–272.

Birkhead T R, Moller A P. 1998. Sperm Competition and Sexual Selection. Academic Press, London.

Borrell Y J, Álvarez J, Vázquez E, Fernández C, Mártinez C, Sánchez J A, Blanco G. 2004. Applying microsatellites to the management of farmed turbot stocks (Scophthalmus maximus L.) in hatcheries. Aquaculture, 241: 133–150.

Borrell Y J, Blanco G, Vázquez E, Piñera J A, Giménez G, Estévez A, Sánchez J A. 2008. Assessing the spawning season in common dentex (Dentex dentex) using microsatellites. Aquac. Res., 39(12): 1 258–1 267.

Boudry P, Collet B, Cornette F, Hervouet V, Bonhomme F. 2002. High variance in reproductive success of the Pacific oyster (Crassostrea gigas, Thunberg) revealed by microsatellite based parentage analysis of multifactorial crosses. Aquaculture, 204: 283–296.

Brown R C, Woolliams J A, McAndrew B J. 2005. Factors influencing effective population size in commercial populations of gilthead seabream, Sparus aurata. Aquaculture, 247: 219–225.

Cross T F, King R D. 1983. Genetic effects of hatchery rearing in Atlantic salmon. Aquaculture, 33: 33–40.

Eknath A E, Doyle R W. 1990. Effective population size and rate of inbreeding in aquaculture of Indian major carps. Aquaculture, 85: 293–305.

Frankham R. 1995. Effective population size/adult population size ratios in wildlife: a review. Genet. Res., 66: 95–107.

Gaffney P M, Davis C V, Hawes R O. 1992. Assessment of drift and selection in hatchery populations of oysters (Crassostrea virginica). Aquaculture, 105: 1–20.

Gamfeldt L, Kallstrom B. 2007. Increasing intraspecific diversity increases predictability in population survival in the face of perturbations. Oikos, 116: 700–705.

Hartl D L. 2000. A Primer of Population Genetics. Sinauer Associates, Sunderland, UK.

Hedgecock D, Chow V, Waples R S. 1992. Effective population numbers of shellfish broodstocks estimated from temporal variance in allelic frequencies. Aquaculture, 108: 215–232.

Hedgecock D, Sly F. 1990. Genetic drift and effective population sizes of hatchery-propagated stocks of the Pacific oyster, Crassostrea gigas. Aquaculture, 88: 21–38.

Hedgecock D. 1994. Does variance in reproductive success limit effective population sizes of marine organisms? In: Beaumont A R ed. Genetics and evolution of aquatic organisms. Chapman and Hall, London, UK. p.122–134.

Herbinger C M, O’Reilly P T, Verspoor E. 2006. Unraveling first-generation pedigrees in wild endangered salmon populations using molecular genetic markers. Mol. Ecol., 15: 2 261–2 275.

Herlin M, Delghandi M, Wesmajervi M, Taggart J B, McAndrew B J, Penman D J. 2008. Analysis of the parental contribution to a group of fry from a single day of spawning from a commercial Atlantic cod (Gadus morhua) breeding tank. Aquaculture, 274: 218–224.

Huan P, Wang H X, Liu B Z. 2012. Transcriptomic analysis of the clam Meretrix meretrix on different larval stages. Mar. Biotechnol., 14: 69–78.

Jackson T R, Martin-Robichaud D J, Reith M E. 2003. Application of DNA markers to the management of Atlantic halibut (Hippoglossus hippoglossus) broodstock. Aquaculture, 220: 245–259.

Kalinowski S T, Taper M L, Marshall T C. 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol. Ecol., 16: 1 099–1 106.

Kim S G, Morishima K, Satoh N, Fujioka T, Saito A, Arai K. 2007. Parentage assignment in hatchery population of brown sole Pleuronectes herzensteini by microsatellite DNA markers. Fish. Sci., 73: 1 087–1 093.

Lallias D, Taris N, Boudry P, Bonhomme F, Lapegue S. 2010. Variance in the reproductive success of flat oyster Ostrea edulis L. assessed by parentage analyses in natural and experimental conditions. Genet. Res. Camb., 92: 175–187.

Lande R, Barrowclough G F. 1987. Effective population size, genetic variation, and their use in population management. In: Soule M ed. Viable Populations for Conservation. Cambridge University Press, Cambridge, UK. p.87–124.

Lemay M A, Boulding E G. 2009. Microsatellite pedigree analysis reveals high variance in reproductive success and reduced genetic diversity in hatchery-spawned northern abalone. Aquaculture, 295: 22–29.

Leonard J L. 2006. Sexual selection: lessons from hermaphrodite mating systems. Integr. Comp. Biol., 46: 349–367.

Lu X, Wang H X, Dai P, Liu B Z. 2011b. Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix. Conserv. Genet. Resour., 3: 655–658.

Lu X, Wang H X, Liu B Z. 2011a. An effective method for parentage determination of the clam (Meretrix meretrix) based on SSR and CO1 markers. Aquaculture, 318: 223–228.

Lynch M, Ritland K. 1999. Estimation of pairwise relatedness with molecular markers. Genetics, 152: 1 753–1 766.

Marshall T C, Slate J, Kruuk L E B, Pemberton J M. 1998. Statistical confidence for likelihood-based paternity inference in natural populations. Mol. Ecol., 7: 639–655.

McGoldrick D J, Hedgecock D, English L J, Baoprasertkul P, Ward R D. 2000. The transmission of microsatellite alleles in Australian and north American stocks of the Pacific oyster (Crassostrea gigas): selection and null alleles. J. Shell. Res., 19: 779–788.

Mcgoldrick D J, Hedgecock D. 1997. Fixation, segregation and linkage of allozyme loci in inbred families of the pacific oyster Crassostrea gigas (Thunberg): implications for the causes of inbreeding depression. Genetics, 146: 321–334.

Norris A T, Bradley D G, Cunningham E P. 2000. Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers. Aquaculture, 182: 73–83.

Notter D R. 1999. The importance of genetic diversity in livestock populations of the future. J. Anim. Sci., 77: 61–69.

Ponzoni R W, Khaw H L, Nguyen N H, Hamzah A. 2010. Inbreeding and effective population size in the Malaysian nucleus of the GIFT strain of Nile tilapia (Oreochromis niloticus). Aquaculture, 302: 42–48.

Porta J, Porta J M, Martinez-Rodriguez G, Alvarez M D C. 2006. Development of a microsatellite multiplex PCR for Senegalese sole (Solea senegalensis) and its application to broodstock management. Aquaculture, 256: 159–166.

Ryman N, Utter F, Laikre L. 1995. Protection of intraspecific biodiversity of exploited fishes. Rev. Fish Biol. Fisher., 5: 417–446.

Saavedra C. 1997. Low effective sizes in hatchery populations of the European Oyster (Ostrea edulis): implications for the management of genetic resources. J. Shellfish Res., 16: 441–446.

Sambrook J, Fritsch E F, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.

Sbordoni V, De Matthaeis E, Cobolli Sbordoni M, La Rosa G, Mattoccia M. 1986. Bottleneck effects and the depression of genetic variability in hatchery stocks of Penaeus japonicus (Crustacea, Decapoda). Aquaculture, 57: 239–251.

Tang B J, Liu B Z, Wang G D, Zhang T, Xiang J H. 2006. Effects of various algal diets and starvation on larval growth and survival of Meretrix meretrix. Aquaculture, 254: 526–533.

Wang H X, Chai X L, Liu B Z. 2011. Estimation of genetic parameters for growth traits in cultured clam Meretrix meretrix (Bivalvia: Veneridae) using the Bayesian method based on Gibbs sampling. Aquac. Res., 42: 240–247.

Wedekind C. 2002. Manipulating sex ratios for conservation: short-term risks and long-term benefits. Anim. Conserv., 5: 13–20.

Wilson A J, Ferguson M M. 2002. Molecular pedigree analysis in natural populations of fishes: approaches, applications, and practical considerations. Can. J. Fish. Aquat. Sci., 59: 1 696–1 707.

Wright S. 1931. Evolution in mendelian genetics. Genetics, 16: 97–159.

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