Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris

Ruijuan Ma; Xiangzhi Lin

doi:10.1007/s00343-014-3142-2

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Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris

Updated：2023-05-16

- Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris
- Journal of Oceanology and Limnology Vol. 32, Issue 2, Pages: 390-396(2014)
- Affiliations：
  
  Engineering Research Center of Marine Biological Resource Comprehensive Utilization Third Institute of Oceanography State Oceanic Administration
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3142-2
  CLC：
- Online First：06 March 2014，
  
  Published：2014
- Accepted：
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Ma, R., Lin, X. , Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris, ., Chin. J. Ocean. Limnol. 32, 390–396 (2014). https://doi.org/10.1007/s00343-014-3142-2
DOI：

Ma, R., Lin, X. , Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris, ., Chin. J. Ocean. Limnol. 32, 390–396 (2014). https://doi.org/10.1007/s00343-014-3142-2 DOI：

摘要

Abstract

Vitreoscilla hemoglobin is an oxygen-binding protein that promotes oxygen delivery and reduces oxygen consumption under low oxygen conditions to increase the efficiency of cell respiration and metabolism. In this study

we introduced a Vitreoscilla hemoglobin gene (vgb) into Chlorella vulgaris by Agrobacterium tumefaciens -mediated transformation (ATMT). PCR analysis confirmed that the vgb gene was successfully integrated into the Chlorella vulgaris genome. Analysis of biomass obtained in shake flasks revealed transformant biomass concentrations as high as 3.28 g/L

which was 38.81% higher than that of the wild-type strain. Lutein content of transformants also increased slightly. Further experiments recovered a maximum lutein yield of 2.91 mg/L from the transformants

which was 36.77% higher than that of the wild-type strain. The above results suggest that integrated expression of the vgb gene may improve cell growth and lutein yield in Chlorella vulgaris

with applications to lutein production from Chlorella during fermentation.

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references

Anila N, Chandrashekar A, Ravishankar G A, Sarada R. 2011. Establishment of Agrobacterium tumefaciens-mediated genetic transformation in Dunaliella bardawil. European Journal of Phycology, 46(1): 36–44.

Bundock P, den Dulk-Ras A, Beijersbergen A, Hooykaas P J. 1995. Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO Journal, 14(13): 3 206–3 214.

Carpentier S, Knaus M, Suh M. 2009. Associations between lutein, zeaxanthin, and age-related macular degeneration: an overview. Critical Reviews in Food Science and Nutrition, 49(4): 313–326.

Cha T S, Chen C F, Yee W, Aziz A, Loh S H. 2011. Cinnamic acid, coumarin and vanillin: alternative phenolic compounds for efficient Agrobacterium -mediated transformation of the unicellular green alga, Nannochloropsis sp. Journal of Microbiological Methods, 84(3): 430–434.

Cha T S, Yee W, Aziz A. 2012. Assessment of factors affecting Agrobacterium -mediated genetic transformation of the unicellular green alga, Chlorella vulgari s. World Journal of Microbiology and Biotechnology, 28(4): 1 771–1 779.

Chen H X, Chu J, Zhang S L, Zhuang Y P, Qian J C, Wang Y H, Hu X Q. 2007. Intracellular expression of Vitreoscilla hemoglobin improves S-adenosylmethionine production in a recombinant Pichia pastoris. Applied Microbiology and Biotechnology, 74(6): 1 205–1 212.

Cheng R B, Ma R J, Li K, Rong H, Lin X Z, Wang Z K, Yang S J, Ma Y. 2012. Agrobacterium tumefaciens mediated transformation of marine microalgae Schizochytrium. Microbiological Research, 167(3): 179–186.

Dwyer J H, Navab M, Dwyer K M, Hassan K, Sun P, Shircore A, Hama-Levy S, Hough G, Wang X P, Drake T, Merz C N B, Fogelman A M. 2001. Oxygenated carotenoid lutein and progression of early atherosclerosis: the Los Angeles atherosclerosis study. Circulation, 103(24): 2 922–2 927.

Feng L, Chen S W, Sun M, Yu Z N. 2007. Expression of Vitreoscilla hemoglobin in Bacillus thuringiensis improve the cell density and insecticidal crystal proteins yield. Applied Microbiology and Biotechnology, 74(2): 390–397.

Fernandez-Sevilla J M, Acien Fernandez F G, Molina Grima E. 2010. Biotechnological production of lutein and its applications. Applied Microbiology and Biotechnology, 86(1): 27–40.

Gouka R J, Gerk C, Hooykaas P J J, Bundock P, Musters W, Verrips C T, de Groot M J A. 1999. Transformation of Aspergillus awamori by Agrobacterium tumefaciensmediated homologous recombination. Nature Biotechnology, 17(6): 598–601.

Hawkins R L, Nakamura M. 1999. Expression of human growth hormone by the eukaryotic alga, Chlorella. Current Microbiology, 38(6): 335–341.

Hooykaas P J J, Roobol C, Schilperoort R A. 1979. Regulation of the transfer of Ti-plasmids of Agrobacterium tumefaciens. Journal of General Microbiology, 110(1): 99–109.

Kathiresan S, Chandrashekar A, Ravishankar G A, Sarada R. 2009. Agrobacterium -mediated transformation in the green alga Haematococcus pluvialis (Chlorophyceae, Volvocales). Journal of Phycology, 45(3): 642–649.

Kumar S V, Misquitta R W, Reddy V S, Rao B J, Rajam M V. 2004. Genetic transformation of the green alga-Chlamydomonas reinhardtii by Agrobacterium tumefaciens. Plant Science, 166(3): 731–738.

Lippmeier J C, Crawford K S, Owen C B, Rivas A A, Metz J G, Apt K E. 2009. Characterization of both polyunsaturated fatty acid biosynthetic pathways in Schizochytrium sp. Lipids, 44(7): 621–630.

Pei Y X, Heng Z C, Duan G C, Wang M C. 2007. The mechanisms and effects of lutein on inducing the cell differentiation of human esophagus cancer EC9706. Journal of Sichuan University, Medical Science Edition, 38(4): 629–632.

Shen W J, Forde B G. 1989. Efficient transformation of Agrobacterium spp. by high voltage electroporation. Nucleic Acids Research, 17(20): 83–85.

Shi X M, Chen F. 1999. Production and vapid extraction of lutein and the other lipid-soluble pigments from Chlorella protothecoides grown under heterotrophic and mixotrophic conditions. Nahrung -Food, 43(2): 109–113.

Staats C C, Junges A, Fitarelli M, Furlaneto M C, Vainstein M H, Schrank A. 2007. Gene inactivation mediated by Agrobacterium tumefaciens in the filamentous fungi Metarhizium anisopliae. Applied Microbiology and Biotechnology, 76(4): 945–950.

Su Y S, Li X, Liu Q Z, Hou Z W, Zhu X Q, Guo X P, Ling P X. 2010. Improved poly-gamma-glutamic acid production by chromosomal integration of the Vitreoscilla hemoglobin gene (vgb) in Bacillus subtilis. Bioresource Technology, 101(12): 4 733–4 736.

Suthar D H, Chattoo B B. 2006. Expression of Vitreoscilla hemoglobin enhances growth and levels of alpha-amylase in Schwanniomyces occidentalis. Applied Microbiology and Biotechnology, 72(1): 94–102.

Wang C H, Wang Y Y, Su Q, Gao X R. 2007. Transient expression of the GUS gene in a unicellular marine green alga, Chlorella sp. MACC/C95, via electroporation. Biotechnology and Bioprocess Engineering, 12(2): 180–183.

Wang X F, Sun Y C, Shen X G, Ke F, Zhao H Y, Liu Y, Xu L, Yan Y J. 2012. Intracellular expression of Vitreoscilla hemoglobin improves production of Yarrowia lipolytica lipase LIP2 in a recombinant Pichia pastoris. Enzyme and Microbial Technology, 50(1): 22–28.

Zhang L, Li Y J, Wang Z N, Xia Y, Chen W S, Tang K X. 2007. Recent developments and future prospects of Vitreoscilla hemoglobin application in metabolic engineering. Biotechnology Advances, 25(2): 123–136.

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