Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)

Jing Li; Shaojun Pang; Tifeng Shan; Feng Liu; Suqin Gao

doi:10.1007/s00343-014-3139-x

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Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)

Updated：2023-05-16

- Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)
- Journal of Oceanology and Limnology Vol. 32, Issue 2, Pages: 365-371(2014)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3139-x
  CLC：
- Online First：28 December 2013，
  
  Published：2014
- Accepted：
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Li, J., Pang, S., Shan, T. et al. Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)., Chin. J. Ocean. Limnol. 32, 365–371 (2014). https://doi.org/10.1007/s00343-014-3139-x
DOI：

Li, J., Pang, S., Shan, T. et al. Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)., Chin. J. Ocean. Limnol. 32, 365–371 (2014). https://doi.org/10.1007/s00343-014-3139-x DOI：

摘要

Abstract

The annual life cycle of the brown seaweed Undaria pinnatifida (Harvey) Suringer comprises a macroscopic diploid sporophyte stage and a microscopic haploid gametophyte stage. In 2011

an unusual zoospore-derived monoecious gametophyte isolate (designated as line 10-5-3) of U. pinnatifida was observed. To understand this phenomenon

a comprehensive screening of eighty-two previously identified male gametophyte cultures

isolated from three randomly selected cultivars (lines 10

and 5) was performed. Thirty-six of the isolates developed both antheridia and oogonia on the same filamentous fragment in a standard gametogenesis test (SGT: 18°C

60 μmol photons/(m2·s)). Selfing of the monoecious gametophyte or crossing it with a normal male gametophyte both gave rise to morphologically normal sporophytic offspring. However

crossing resulted in a much higher fertilization rate (89.7%). The hybrid and selfed sporophytic offspring were grown to maturity in flow tanks at an ambient temperature of 10–18°C over a period of 69 days. Active zoospores were released from both types of mature sporophylls. The majority of these developed into male gametophytes

while 15%–20% developed into the observed monoecious structures on the same filament. Using PCR amplification it was found that all the monoecious gametophyte isolates and the sporophytic offspring resulting from the selfing and crossing lacked the femalelinked microsatellite sequence (a part of the locus Up-AC-2A8

GenBank accession No. AY738602.1)

indicating their male nature. U. pinnatifida is an invasive species in some regions and the implications of the above findings for this species in nature are briefly discussed.

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references

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