Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)

Zhanru Shao; Fuli Liu; Qiuying Li; Jianting Yao; Delin Duan

doi:10.1007/s00343-014-3130-6

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Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)

Updated：2023-05-16

- Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)
- Journal of Oceanology and Limnology Vol. 32, Issue 2, Pages: 377-389(2014)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3130-6
  CLC：
- Online First：28 December 2013，
  
  Published：2014
- Accepted：
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Shao, Z., Liu, F., Li, Q. et al. Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)., Chin. J. Ocean. Limnol. 32, 377–389 (2014). https://doi.org/10.1007/s00343-014-3130-6
DOI：

Shao, Z., Liu, F., Li, Q. et al. Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)., Chin. J. Ocean. Limnol. 32, 377–389 (2014). https://doi.org/10.1007/s00343-014-3130-6 DOI：

摘要

Abstract

Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments

and consequently may have highly efficient ribulose-1

5-bisphosphate carboxylase/oxygenase (Rubisco) activity for carbon assimilation. In our study

we cloned the full-length Rubisco gene from S. japonica (SJ-rbc). It contained an open reading frame for a large subunit gene (SJ — rbcL) of 1 467 bp

a small subunit gene (SJ-rbcS) of 420 bp

and a SJ-rbcL/S intergenic spacer of 269 bp. The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa

5.81 and 15.84 kDa

4.71

respectively. After induction with 1 mmol/L isopropyl-β-D-thiogalactopyranoside for 5 h and purification by Ni2+ affinity chromatography

electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein. Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night. This observation implied that Rubisco played a role in normal gametophytic growth and development. In juvenile sporophytes

mRNA levels of SJ-rbcL

carbonic anhydrase

Calvin-Benson-Bassham cycle-related enzyme

and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance. Similarly

expression of these genes was up-regulated under blue light irradiance at 350 μmol/(m2·s). Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements.

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