Primers to block the amplification of symbiotic apostome ciliate 18S rRNA gene in a PCR-based copepod diet study

Xiaoyan Yi; Huan Zhang; Guangxing Liu

doi:10.1007/s00343-014-3129-z

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Primers to block the amplification of symbiotic apostome ciliate 18S rRNA gene in a PCR-based copepod diet study

Updated：2023-05-16

- Primers to block the amplification of symbiotic apostome ciliate 18S rRNA gene in a PCR-based copepod diet study
- Journal of Oceanology and Limnology Vol. 32, Issue 3, Pages: 515-521(2014)
- Affiliations：
  
  1. College of Environmental Science and Engineering Ocean University of China
  2. Department of Marine Sciences Connecticut University
  3. Ministry of Education Key Laboratory of Marine Environment and Ecology (Ocean University of China)
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3129-z
  CLC：
- Online First：04 May 2014，
  
  Published：2014
- Accepted：
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Yi, X., Zhang, H. & Liu, G. Primers to block the amplification of symbiotic apostome ciliate 18S rRNA gene in a PCR-based copepod diet study., Chin. J. Ocean. Limnol. 32, 515–521 (2014). https://doi.org/10.1007/s00343-014-3129-z
DOI：

Yi, X., Zhang, H. & Liu, G. Primers to block the amplification of symbiotic apostome ciliate 18S rRNA gene in a PCR-based copepod diet study., Chin. J. Ocean. Limnol. 32, 515–521 (2014). https://doi.org/10.1007/s00343-014-3129-z DOI：

摘要

Abstract

Pelagic copepods play an important role in the marine food web. However

a full understanding of the ecological status of this zooplankton group depends on the careful study of their natural diets. In previous PCR-based copepod diet studies

we found many apostome ciliates that live symbiotically under the exoskeleton of the copepods

and their sequences were often over-represented in the 18S rRNA gene (18S rDNA) libraries. As a first step to address this issue

we designed three apostome ciliate 18S rDNA blocking primers

and tested their blocking efficiency against apostome ciliate 18s rDNA under various PCR conditions. Using a semi-quantitative PCR method

we optimized the conditions to efficiently amplify the 18S rDNA of the prey while simultaneously excluding the symbiotic apostome ciliates. This technique will facilitate PCR-based diet studies of copepods and other zooplankton in their natural environments.

关键词

Keywords

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