Effect of Fe3+ on the growth and lipid content of Isochrysis galbana

Zhiyuan Liu; Guangce Wang

doi:10.1007/s00343-014-3110-x

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Effect of Fe3+ on the growth and lipid content of Isochrysis galbana

Updated：2023-05-16

- Effect of Fe3+ on the growth and lipid content of Isochrysis galbana
- Journal of Oceanology and Limnology Vol. 32, Issue 1, Pages: 47-53(2014)
- Affiliations：
  
  1. College of Oceanology Hainan University
  2. Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3110-x
  CLC：
- Online First：21 February 2014，
  
  Published：2014
- Accepted：
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Liu, Z., Wang, G. Effect of Fe3+ on the growth and lipid content of Isochrysis galbana, ., Chin. J. Ocean. Limnol. 32, 47–53 (2014). https://doi.org/10.1007/s00343-014-3110-x
DOI：

Liu, Z., Wang, G. Effect of Fe3+ on the growth and lipid content of Isochrysis galbana, ., Chin. J. Ocean. Limnol. 32, 47–53 (2014). https://doi.org/10.1007/s00343-014-3110-x DOI：

摘要

Abstract

Inducing lipid accumulation in microalgae cells without suppressing cell growth is vital to the economical production of biodiesel from microalgae. In two experiments

we demonstrate that the cell concentration and lipid content of marine microalgae Isochrysis galbana depend upon the iron concentration in the growth media. In Experiment I

adding chelated FeCl3 to the medium at the late exponential growth phase prolonged this phase and increased the lipid content in I. galbana cells. The final cell density and lipid content of I. galbana supplemented with chelated FeCl3 was approximately 2 and 1.65 times higher than that of non-supplemented cultures

respectively. In Experiment II

I. galbana cells in the late exponential phase were collected and re-inoculated into new media containing Fe3+ at various concentrations. The final cell concentration and lipid content were maximized at the highest iron concentration (38% biomass by dry weight at 1.2×10−5 mol/L Fe3+). In this study

intracellular neutral lipid storage was evaluated by fluorescent spectrophotometry using fluorochrome Nile red

and the measurement conditions were optimized.

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