Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus

Mengxin Xing; Zhanhui Hou; Yanmei Qu; Bin Liu

doi:10.1007/s00343-014-3099-1

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Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus

Updated：2023-05-16

- Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus
- Journal of Oceanology and Limnology Vol. 32, Issue 2, Pages: 316-325(2014)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3099-1
  CLC：
- Online First：06 March 2014，
  
  Published：2014
- Accepted：
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Xing, M., Hou, Z., Qu, Y. et al. Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus, ., Chin. J. Ocean. Limnol. 32, 316–325 (2014). https://doi.org/10.1007/s00343-014-3099-1
DOI：

Xing, M., Hou, Z., Qu, Y. et al. Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus, ., Chin. J. Ocean. Limnol. 32, 316–325 (2014). https://doi.org/10.1007/s00343-014-3099-1 DOI：

摘要

Abstract

Eighteen agar media were tested for the culture of gut-associated bacteria from farmed adult turbot (Scophthalmus maximus)

including 16 agar media with or without 1% gastrointestinal (GI) supernatant

or with 2% or 4% GI supernatant. A total of 1 711 colonies were analyzed and 24 operational taxonomic units (OTUs) were identified. The greatest bacterial diversity was isolated on Zobell 2216E/Zobell 2216E+ agar media

whereas MRS/MRS+ agar media produced a low diversity of colonies. Agar media with GI supernatant (1%

or 4%) showed increased diversity and yielded different profiles of OTUs from the corresponding original media

suggesting that GI supernatant provides substances that enhance the culture efficiency of bacteria from the turbot GI tract. The large majority of the colonies (82%) were γ-Proteobacteria

whereas 15.6% and 2.4% of colonies were Firmicutes and Actinobacteria

respectively. At the genus level

49.4% of all colonies were assigned to Vibrio. Other potential pathogens

including Pseudomonas

Photobacterium

and Enterobacter

and potential probiotics

including Bacillus

Paenibacillus

and Pseudomonas

were also isolated on agar media. Most cultured bacteria belonged to species that were first described in the turbot GI tract. The impact of these species on turbot physiology and health should be investigated further.

关键词

Keywords

references

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