Spatiotemporal pattern of bacterioplankton in Donghu Lake

Xiang Zhang; Qingyun Yan; Yuhe Yu; Lili Dai

doi:10.1007/s00343-014-3037-2

Your Location：

Home >

Browse articles >

Spatiotemporal pattern of bacterioplankton in Donghu Lake

Updated：2023-05-16

- Spatiotemporal pattern of bacterioplankton in Donghu Lake
- Journal of Oceanology and Limnology Vol. 32, Issue 3, Pages: 554-564(2014)
- Affiliations：
  
  1. Key Laboratory of Biodiversity and Conservation of Aquatic Organisms Institute of Hydrobiology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3037-2
  CLC：
- Online First：04 May 2014，
  
  Published：2014
- Accepted：
Scan QR Code
Zhang, X., Yan, Q., Yu, Y. et al. Spatiotemporal pattern of bacterioplankton in Donghu Lake., Chin. J. Ocean. Limnol. 32, 554–564 (2014). https://doi.org/10.1007/s00343-014-3037-2
DOI：

Zhang, X., Yan, Q., Yu, Y. et al. Spatiotemporal pattern of bacterioplankton in Donghu Lake., Chin. J. Ocean. Limnol. 32, 554–564 (2014). https://doi.org/10.1007/s00343-014-3037-2 DOI：

摘要

Abstract

Bacterioplankton play key roles in the biogeochemical cycle and in organic contaminant degradation. The species richness and abundance of bacterial subgroups are generally distinct from each other

and this is attributed to their different functions in aquatic ecosystems. The spatiotemporal variations of eight phylogenetic subgroups (Actinobacteria

Bacteroidetes

Cyanobacteria

Firmicutes

Planctomycetes

alpha-

beta-

and gamma-Proteobacteria) derived from Donghu Lake were investigated using PCR-DGGE fingerprinting

to explore their responses to environmental factors. Results indicate that Actinobacteria and beta-Proteobacteria were the two largest bacterial subgroups detected. These two groups and Bacteroidetes showed clear seasonal patterns in composition of the operational taxonomic unit. Results also suggest that the bacterioplankton subgroups in Donghu Lake were significantly correlated with different environmental factors. In brief

the total nitrogen was one of the major factors regulating all the bacterioplankton except for Actinobacteria. However

total phosphorus

another important eutrophication factor

contributed to the two largest bacterial groups (Actinobacteria and beta-Proteobacteria)

as well as to the Cyanobacteria and Firmicutes. Therefore

the responses of bacterioplankton subgroups to environmental factors were different

and this should be attributed to the differences in the functions of different groups.

关键词

Keywords

references

Allgaier M, Grossart H-P. 2006. Diversity and seasonal dynamics of Actinobacteria populations in four lakes in northeastern Germany. Appl. Environ. Microbiol., 72(5): 3 489–3 497.

Allgaier M, Brückner S, Jaspers E, Grossart H-P. 2007. Intra- and inter-lake variability of free-living and particleassociated Actinobacteria communities. Environ. Microbiol., 9(11): 2 728–2 741.

Andreatta S, Wallinger M M, Piera J, Catalan J, Psenner R, Hofer J S, Sommaruga R. 2004. Tools for discrimination and analysis of lake bacterioplankton subgroups measured by flow cytometry in a high-resolution depth profile. Aquat. Microb. Ecol., 36(2): 107–115.

Ashelford K E, Weightman A J, Fry J C. 2002. PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database. Nucleic Acids Res., 30(15): 3 481–3 489.

Blackwood C B, Oaks A, Buyer J S. 2005. Phylum- and class-specific PCR primers for general microbial community analysis. Appl. Environ. Microbiol., 71(10): 6 193–6 198.

Boon N, De Windt W, Verstraete W, Top E M. 2002. Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. FEMS Microbiol. Ecol., 39(2): 101–112.

Buckley D H, Huangyutitham V, Nelson T A, Rumberger A, Thies J E. 2006. Diversity of Planctomycetes in soil in relation to soil history and environmental heterogeneity. Appl. Environ. Microbiol., 72(7): 4 522–4 531.

Crump B C, Kling G W, Bahr M, Hobbie J E. 2003. Bacterioplankton community shifts in an Arctic lake correlate with seasonal changes in organic matter source. Appl. Environ. Microbiol., 69(4): 2 253–2 268.

Dorigo U, Volatier L, Humbert J F. 2005. Molecular approaches to the assessment of biodiversity in aquatic microbial communities. Water Res., 39(11): 2 207–2 218.

Fromin N, Hamelin J, Tarnawski S, Roesti D, Jourdain-Miserez K, Forestier N, Teyssier-Cuvelle S, Gillet F, Aragno M, Rossi P. 2002. Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns. Environ. Microbiol., 4(11): 634–643.

Glöckner F O, Zaichikov E, Belkova N, Denissova L, Pernthaler J, Pernthaler A, Amann R. 2000. Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of Actinobacteria. Appl. Environ. Microbiol., 66(11): 5 053–5 065.

Haakensen M, Dobson C M, Deneer H, Ziola B. 2008. Realtime PCR detection of bacteria belonging to the Firmicutes Phylum. Int. J. Food Microbiol., 125(3): 236–241.

Holmfeldt K, Dziallas C, Titelman J, Pohlmann K, Grossart H-P, Riemann L. 2009. Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea. Environ. Microbiol., 11(8): 2 042–2 054.

Jetten M S M. 2008. The microbial nitrogen cycle. Environ. Microbiol., 10(11): 2 903–2 909.

Kemp P F, Aller J Y. 2004. Bacterial diversity in aquatic and other environments: what 16S rDNA libraries can tell us. FEMS Microbiol. Ecol., 47(2): 161–177.

Kersters K, De Vos P, Gillis M, Swings J, Vandamme P, Stackebrandt E. 2006 Introduction to the Proteobacteria. In: Dwarkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E eds. The Prokaryotes. Springer, New York. p.3–37.

Kondo R, Nakagawa A, Mochizuki L, Osawa K, Fujioka Y, Butani J. 2009. Dominant bacterioplankton populations in the meromictic Lake Suigetsu as determined by denaturing gradient gel electrophoresis of 16S rRNA gene fragments. Limnology, 10(1): 63–69.

Laque T, Farjalla V F, Rosado A S, Esteves F A. 2010. Spatiotemporal variation of bacterial community composition and possible controlling factors in tropical shallow lagoons. Microb. Ecol., 59(4): 819–829.

Lee S H, Malone C, Kemp P F. 1993. Use of multiple 16S rRNA-targeted fluorescent probes to increase signal strength and measure cellular RNA from natural planktonic bacteria. Mar. Ecol. Prog. Ser., 101(1–2): 193–201.

Lepš J, Šmilauer P. 2003. Multivariate Analysis of Ecological Data Using CANOCO. Cambridge University Press, Cambridge.

Lindström E S. 2001. Investigating infl uential factors on bacterioplankton community composition: results from a field study of five mesotrophic lakes. Microb. Ecol., 42(4): 598–605.

Mühling M, Woolven-Allen J, Murrell J C, Joint I. 2008. Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities. ISME J., 2(4): 379–392.

Martínez-Alonso M, Méndez-Álvarez S, Ramírez-Moreno S, González-Toril E, Amils R, Gaju N. 2008. Spatial heterogeneity of bacterial populations in monomictic Lake Estanya (Huesca, Spain). Microb. Ecol., 55(4): 737–750.

Mueller-Spitz S R, Goetz G W, McLellan S L. 2009. Temporal and spatial variability in nearshore bacterioplankton communities of Lake Michigan. FEMS Microbiol. Ecol., 67(3): 511–522.

Muyzer G, de Waal E C, Uitterlinden A G. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reactionamplified genes coding for 16S rRNA. Appl. Environ. Microbiol., 59(3): 695–700.

Neef A, Amann R, Schlesner H, Schleifer K-H. 1998. Monitoring a widespread bacterial group: in situ detection of planctomycetes with 16S rRNA-targeted probes. Microbiology, 144: 3 257–3 266.

Newton R J, Jones S E, Helmus M R, McMahon K D. 2007. Phylogenetic ecology of the freshwater Actinobacteria acI lineage. Appl. Environ. Microbiol., 73(22): 7 169–7 176.

Nishimura Y, Kim C, Nagata T. 2005. Vertical and seasonal variations of bacterioplankton subgroups with different nucleic acid contents: possible regulation by phosphorus. Appl. Environ. Microbiol., 71(10): 5 828–5 836.

Øvreås L, Forney L, Daae F L, Torsvik V. 1997. Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA. Appl. Environ. Microbiol., 63(9): 3 367–3 373.

Pearce D A. 2003. Bacterioplankton community structure in a maritime Antarctic oligotrophic lake during a period of holomixis, as determined by denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH). Microb. Ecol., 46(1): 92–105.

Reche I, Pulido-Villena E, Morales-Baquero R, Casamayor E O. 2005. Does ecosystem size determine aquatic bacterial richness?. Ecology, 86(7): 1 715–1 722.

Sánchez O, Gasol J M, Massana R, Mas J, Pedrós-Alió C. 2007. Comparison of different denaturing gradient gel electrophoresis primer sets for the study of marine bacterioplankton communities. Appl. Environ. Microbiol., 73(18): 5 962–5 967.

Schiaffino M R, Unrein F, Gasol J M, Farias M E, Estevez C, Balagué V, Izaguirre I. 2009. Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status. Polar Biol., 32(6): 923–936.

Sommaruga R, Casamayor E O. 2009. Bacterial “cosmopolitanism” and importance of local environmental factors for community composition in remote high altitude lakes. Freshwater Biol., 54(5): 994–1 005.

Stach J E M, Maldonado L A, Ward A C, Goodfellow M, Bull A T. 2003. New primers for the class Actinobacteria: application to marine and terrestrial environments. Environ. Microbiol., 5(10): 828–841.

van der Gucht K, Vandekerckhove T, Vloemans N, Cousin S, Muylaert K, Sabbe K, Gillis M, Declerk S, de Meester L, Vyverman W. 2005. Characterization of bacterial communities in four freshwater lakes differing in nutrient load and food web structure. FEMS Microbiol. Ecol., 53(2): 205–220.

Yan Q Y, Yu Y H, Feng W S, Deng W N, Song X H. 2007. Genetic diversity of plankton community as depicted by PCR-DGGE fingerprinting and its relation to morphological composition and environmental factors in Lake Donghu. Microb. Ecol., 54(2): 290–297.

Yan Q Y, Yu Y H, Feng W S, Pan G, Chen H, Chen J, Yang B, Li X M, Zhang X. 2009. Plankton community succession in artificial systems subjected to cyanobacterial blooms removal using chitosan-modified soils. Microb. Ecol., 58(1): 47–55.

Yu Y H, Yan Q Y, Feng W S. 2008. Spatiotemporal heterogeneity of plankton communities in Lake Donghu, China, as revealed by PCR-denaturing gradient gel electrophoresis and its relation to biotic and abiotic factors. FEMS Microbiol. Ecol., 63(3): 328–337.

Zehr J P, Mellon M T, Zani S. 1998. New nitrogen-fixing microorganisms detected in oligotrophic oceans by amplifi cation of nitrogenase (nifH) genes. Appl. Environ. Microbiol., 64(9): 3 444–3 450.

Zeng J, Yang L Y, Du H W, Xiao L, Jiang L J, Wu J, Wang X L. 2009. Bacterioplankton community structure in a eutrophic lake in relation to water chemistry. World J. Microb. Biot., 25(5): 763–772.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

The effect of cylindrospermopsin on the bacterioplankton community: a microcosm experiment on water from Dishui Lake, Shanghai, China

Molecular analyses of bacterioplankton communities with highly abundant Vibrio clades: a case study in Bohai Sea coastal waters

The ecological risks of hydrogen peroxide as a cyanocide: its effect on the community structure of bacterioplankton

Changes in fish diversity and community structure in the central and southern Yellow Sea from 2003 to 2015

Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment

Related Author

Sicheng YIN

Ruohan HUANG

Erik JEPPESEN

Lijing CHEN

Liqing WANG

Xin FANG

Wei ZHANG

Jia HE

Related Institution

Institute of Marine Sciences, Middle East Technical University, Mersin

Limnology Laboratory and EKOSAM, Department of Biological Sciences, Middle East Technical University

Sino-Danish Centre for Education and Research (SDC), University of Chinese Academy of Sciences

Department of Ecoscience, Aarhus University, Silkeborg

Engineering Research Center of Environmental DNA and Ecological Water Health Assessment, Shanghai Ocean University

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰