Use of calcein and alizarin red S for immersion marking of black rockfish Sebastes schlegelii juveniles

Hongjian Lü; Xiumei Zhang; Dan Xi; Tianxiang Gao

doi:10.1007/s00343-014-3022-9

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Use of calcein and alizarin red S for immersion marking of black rockfish Sebastes schlegelii juveniles

Updated：2023-05-16

- Use of calcein and alizarin red S for immersion marking of black rockfish Sebastes schlegelii juveniles
- Journal of Oceanology and Limnology Vol. 32, Issue 1, Pages: 88-98(2014)
- Affiliations：
  
  1. College of Fisheries Ocean University of China
  2. College of Animal Science and Technology Southwest University
- Author bio：
- Funds：
- DOI：10.1007/s00343-014-3022-9
  CLC：
- Online First：21 February 2014，
  
  Published：2014
- Accepted：
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Lü, H., Zhang, X., Xi, D. et al. Use of calcein and alizarin red S for immersion marking of black rockfish Sebastes schlegelii juveniles., Chin. J. Ocean. Limnol. 32, 88–98 (2014). https://doi.org/10.1007/s00343-014-3022-9
DOI：

Lü, H., Zhang, X., Xi, D. et al. Use of calcein and alizarin red S for immersion marking of black rockfish Sebastes schlegelii juveniles., Chin. J. Ocean. Limnol. 32, 88–98 (2014). https://doi.org/10.1007/s00343-014-3022-9 DOI：

摘要

Abstract

Black rockfish Sebastes schlegelii juveniles (30–40 mm total length) were immersed in a range of calcein (CAL) solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S (ARS) solutions at concentrations ranging from 100 to 500 mg/L in filtered seawater (salinity 30) for 24 h. Fluorescent marks were detected in otoliths (sagittae

asteriscus)

scales

fin rays (dorsal

pectoral

ventral

anal

and caudal fin rays)

and fin spines (dorsal

ventral

and anal fin spines) after a 60-d growth experiment. With the exception of 50–100 mg/L CAL

acceptable marks were produced in the otoliths and fin spines by all concentrations of CAL and ARS. In particular

marks were clearly visible under normal light in the sagittae

asteriscus

and fin spines of fish immersed in 200–500 mg/L

300–500 mg/L

and 200–500 mg/L ARS

respectively. Scales and fin rays had acceptable marks at much higher concentrations (≥50 mg/L CAL

≥300 mg/L ARS for scales and ≥50 mg/L CAL

≥200 mg/L ARS for fin rays). The mark quality was highest (i.e.

acceptable marks were observed in all sampled structures after immersion marking) in fish immersed in 150–250 mg/L CAL or 300–500 mg/L ARS. In addition

there was no significant difference in survival and growth of marked fish compared with controls 60 d post-marking ( P >0.05).

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references

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