A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression

Jiquan Zhang; Jing Wang; Jianhai Xiang

doi:10.1007/s00343-013-3089-8

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A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression

Updated：2023-05-16

- A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression
- Journal of Oceanology and Limnology Vol. 31, Issue 6, Pages: 1204-1209(2013)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-3089-8
  CLC：
- Online First：06 November 2013，
  
  Published：2013
- Accepted：
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Zhang, J., Wang, J. & Xiang, J. A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression., Chin. J. Ocean. Limnol. 31, 1204–1209 (2013). https://doi.org/10.1007/s00343-013-3089-8
DOI：

Zhang, J., Wang, J. & Xiang, J. A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression., Chin. J. Ocean. Limnol. 31, 1204–1209 (2013). https://doi.org/10.1007/s00343-013-3089-8 DOI：

摘要

Abstract

Metallothioneins (MTs) are a group of low molecular weight cysteine-rich proteins capable of binding heavy metal ions. A cadmium metallothionein (EcMT — Cd) cDNA with a 189 bp open reading frame (ORF) that encoded a 62 amino acid protein was obtained from Exopalaemon carinicauda. Seventeen cysteines were in the deduced amino acid sequence

and the cysteine (Cys)-rich characteristic was revealed in different metallothioneins in other species. In addition

the deduced amino acid sequence did not contain any aromatic amino acid residues

such as tyrosine (Tyr)

tryptophan (Trp)

and phenylalanine (Phe). EcMT—Cd mRNA was expressed in all tested tissues (the ovary

muscle

stomach

and hepatopancreas)

and its expression profiles in the hepatopancreas were very different when shrimps were exposed to seawater containing either 50 μmol/L CuSO4 or 2.5 μmol/L CdCl 2. The expression of EcMT-Cd was significantly up-regulated in shrimp exposed to CuSO4 for 12 h and down-regulated in shrimps exposed to CdCl2 for 12 h. After 24 h exposure to both metals

its expression was down-regulated. By contrast

at 48 h the EcMT-Cd was up-regulated in test shrimps exposed to CdCl2. The transcript of EcMT-Cd was very low or even absent before the zoea stage

and the expression of EcMT-Cd was detected from mysis larvae-I

then its expression began to rise. In conclusion

a cadmium MT exists in E. carinicauda that is expressed in different tissues and during different developmental stages

and responds to the challenge with heavy metal ions

which provides a clue to understanding the function of cadmium MT.

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references

Amiard J C, Amiard-Triquet C, Barka S, Pellerin J, Rainbow P S. 2006. Metallothioneins in aquatic invertebrates: their role in metal detoxification and their use as biomarkers. Aquatic Toxicology, 76: 160–202

Andrews G K, Huethudson Y M, Paria B C, Mcmaster M T, De S K, Dey S K. 1991. Metallothionein gene-expression and metal regulation during preimplantation mouse embryo development (Mt messenger-RNA during early development). Dev. Biol., 145: 13–27.

Andrews G K, Lee D K, Ravindra R, Lichtlen P, Sirito M, Sawadogo M, Schaffner W. 2001. The transcription factors MTF-1 and USF1 cooperate to regulate mouse metallothionein-I expression in response to the essential metal zinc in visceral endoderm cells during early development. Embo. J., 20: 1 114–1 122.

Ang S G, Wong V W T. 1991. Chromatographic analysis of metallothionein proteins from the crab species Scylla s errata and Portunus p elagicus. J. Liq. Chromatogr., 14: 2 647–2 663.

Brouwer M, Schlenk D, Ringwood A H, Brouwer-Hoexum T. 1992. Metal-specific induction of metallothionein isoforms in the blue crab Callinectes sapidus in response to single- and mixed-metal exposure. Arch. Biochem. Biophys., 294: 461–468.

Chen W Y, John J A C, Lin C H, Lin H F, Wu S C, Lin C H, Chang C Y. 2004. Expression of metallothionein gene during embryonic and early larval development in zebrafish. Aquat. Toxicol., 69: 215–227.

Kojima Y, Kagi J H R. 1978. Metallothionein. Trends Biochem. Sci., 3: 90–93.

Li J, Han J, Chen P, Chang Z, He Y, Liu P, Wang Q. 2012. Cloning of a heat shock protein 90 (HSP90) gene and expression analysis in the ridgetail white prawn Exopalaemon carinicauda. Fish Shellfish Immunol., 32: 1 191–1 197.

Luan W, Li F H, Zhang J Q, Wen R, Li Y T, Xiang J H. 2010. Identification of a novel inducible cytosolic Hsp70 gene in Chinese shrimp Fenneropenaeus chinensis and comparison of its expression with the cognate Hsc70 under different stresses. Cell Stress Chaperon., 15: 83–93.

Mahmood K, Yang J S, Chen D, Wang M, Yang F, Yang W J. 2009. Response of metallothionein gene-1 to laboratory exposure to heavy metals and thermal stress in the freshwater prawn Macrobrachium rosenbergii. J. Hazard. Mater., 167: 523–530.

Nordberg M, Nordberg G F. 2000. Toxicological aspects of metallothionein. Cell. Mol. Biol., 46: 451–463.

Olsson P E, Zafarullah M, Foster R, Hamor T, Gedamu L. 1990. Developmental regulation of metallothionein messenger-RNA, zinc and copper levels in rainbow trout, Salmo gairdneri. Eur. J. Biochem., 193(1): 229–235.

Pourang N, Dennis J H, Ghourchian H. 2005. Distribution of heavy metals in Penaeus semisulcatus from Persian Gulf and possible role of metallothionein in their redistribution during storage. Environ. Moni. t. Assess., 100: 71–88.

Ren F, Jiang H, Sun J, He L, Li W, Wang Y, Wang Q. 2011. Cloning, characterization, expression, and copper sensitivity of the metallothionein-1 gene in the Chinese mitten crab, Eriocheir sinensis. Mol. Biol. Rep., 38: 2 383–2 393.

Richards M P. 1984. Synthesis of a metallothionein-like protein by developing turkey embryos maintained in long-term, shell-less culture. J. Pediatr. Gastr. Nutr., 3: 128–136.

Russo R, Zito F, Matranga V. 2013. Tissue-specificity and phylogenetics of Pl-MT mRNA during Paracentrotus lividus embryogenesis. Gene, 519: 305–310.

Simoniello P, Motta C M, Scudiero R, Trinchella F, Filosa S. 2010. Spatiotemporal changes in metallothionein gene expression during embryogenesis in the wall lizard Podarcis sicula. J. Exp. Zool. Part A, 313: 410–420.

Soares T M, Coutinho D A, Lacerda L D, Moraes M O, Rebelo M F. 2011. Mercury accumulation and metallothionein expression from aquafeeds by Litopenaeus vannamei Boone, 1931 under intensive aquaculture conditions. Braz. J. Microbiol., 71: 131–137.

Sun Z, Zhang X J, Zhang J Q, Li F H, Zhang J B, Xiang J H. 2012. Comparative transcriptome analysis on embryo and adults of Exopalamon carincauda. The 8 th Symposium of World’s Chinese Scientist’s on Crustacean Aquaculture. p.207.

Syring R A, Hoexum Brouwer T, Brouwer M. 2000. Cloning and sequencing of cDNAs encoding for a novel copperspecific metallothionein and two cadmium-inducible metallothioneins from the blue crab Callinectes sapidus. Comp. Biochem. Physiol. C, 125: 325–332.

Wu J P, Chen H C. 2005. Metallothionein induction and heavy metal accumulation in white shrimp Litopenaeus vannamei exposed to cadmium and zinc. Comp. Biochem. Physiol. C, 140: 383–394.

Yamuna A, Bhavan P S, Geraldine P. 2012. Glutathione S-transferase and metallothionein levels in the freshwater prawn Macrobrachium malcolmsonhi exposed to mercury. J. Environ. Biol., 33: 133–137.

Yang C J, Zhang J Q, Li F H, Ma H M, Zhang Q L, Priya Y A J, Zhang X J, Xiang J H. 2008. A toll receptor from Chinese shrimp Fenneropenaeus chinensis is responsive to Vibrio anguillarum infection. Fish Shellfish Immun., 24: 564–574.

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