Molecular cloning and characterization of SoxB2 gene from Zhikong scallop Chlamys farreri

Yan He; Zhenmin Bao; Huihui Guo; Yueyue Zhang; Lingling Zhang; Shi Wang; Jingjie Hu; Xiaoli Hu

doi:10.1007/s00343-013-3039-5

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Molecular cloning and characterization of SoxB2 gene from Zhikong scallop Chlamys farreri

Updated：2023-05-16

- Molecular cloning and characterization of SoxB2 gene from Zhikong scallop Chlamys farreri
- Journal of Oceanology and Limnology Vol. 31, Issue 6, Pages: 1216-1225(2013)
- Affiliations：
  
  Key Laboratory of Marine Genetics and Breeding (MGB) Ministry of Education College of Marine Life Sciences Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-3039-5
  CLC：
- Online First：06 November 2013，
  
  Published：2013
- Accepted：
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He, Y., Bao, Z., Guo, H. et al. Molecular cloning and characterization of SoxB2 gene from Zhikong scallop Chlamys farreri, ., Chin. J. Ocean. Limnol. 31, 1216–1225 (2013). https://doi.org/10.1007/s00343-013-3039-5
DOI：

He, Y., Bao, Z., Guo, H. et al. Molecular cloning and characterization of SoxB2 gene from Zhikong scallop Chlamys farreri, ., Chin. J. Ocean. Limnol. 31, 1216–1225 (2013). https://doi.org/10.1007/s00343-013-3039-5 DOI：

摘要

Abstract

The Sox proteins play critical roles during the development of animals

including sex determination and central nervous system development. In this study

the SoxB2 gene was cloned from a mollusk

the Zhikong scallop (Chlamys farreri)

and characterized with respect to phylogeny and tissue distribution. The full-length cDNA and genomic DNA sequences of C. farreri SoxB2 (Cf SoxB2) were obtained by rapid amplification of cDNA ends and genome walking

respectively

using a partial cDNA fragment from the highly conserved DNA-binding domain

i.e.

the High Mobility Group (HMG) box. The full-length cDNA sequence of Cf SoxB2 was 2 048 bp and encoded 268 amino acids protein. The genomic sequence was 5 551 bp in length with only one exon. Several conserved elements

such as the TATA-box

GC-box

CAAT-box

GATA-box

and Sox/sry-sex/testis-determining and related HMG box factors

were found in the promoter region. Furthermore

real-time quantitative reverse transcription PCR assays were carried out to assess the mRNA expression of Cf SoxB 2 in different tissues. SoxB2 was highly expressed in the mantle

moderately in the digestive gland and gill

and weakly expressed in the gonad

kidney and adductor muscle. In male and female gonads at different developmental stages of reproduction

the expression levels of Cf SoxB2 were similar. Considering the specific expression and roles of SoxB 2 in other animals

in particular vertebrates

and the fact that there are many pallial nerves in the mantle

cerebral ganglia in the digestive gland and gill nerves in gill

we propose a possible essential role in nervous tissue function for Sox B 2 in C. farreri.

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references

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