First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains

Zhaohe Luo; Weidong Yang; Bin Xu; Haifeng Gu

doi:10.1007/s00343-013-2315-8

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First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains

Updated：2023-05-16

- First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains
- Journal of Oceanology and Limnology Vol. 31, Issue 4, Pages: 835-845(2013)
- Affiliations：
  
  1. College of Life Science and Technology Jinan University
  2. Third Institute of Oceanography SOA
  3. Institute of Urban Environment Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-2315-8
  CLC：
- Online First：10 July 2013，
  
  Published：2013
- Accepted：
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Luo, Z., Yang, W., Xu, B. et al. First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains., Chin. J. Ocean. Limnol. 31, 835–845 (2013). https://doi.org/10.1007/s00343-013-2315-8
DOI：

Luo, Z., Yang, W., Xu, B. et al. First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains., Chin. J. Ocean. Limnol. 31, 835–845 (2013). https://doi.org/10.1007/s00343-013-2315-8 DOI：

摘要

Abstract

The presence of Biecheleria cincta (= Woloszynskia cincta) in the Chinese coasts is reported for the first time. In scanning electron microscope

three to five series of vesicles and an elongated apical vesicle (EAV) were visible in the epicone

and both the hypocone and the cingulum had three series of vesicles each. Thin sections revealed that B. cincta possesses stalked pyrenoids and an unusual eyespot consisting of a stack of cisternae with brick-like materials (type E)

thus supporting its transfer from Woloszynskia to Biecheleria. Spiny cysts formed spontaneously in culture

with an encystment rate of around 20%. Both large subunit ribosomal DNA (LSU rDNA) and internal transcribed spacer region (ITS) sequences in 12 strains from the Chinese coasts were determined. Phylogenetic analyses based on LSU rDNA and ITS sequences using Bayesian inference and maximum likelihood revealed two distinct ribotypes (referred to as ribotype A and B) in B. cincta. ITS region pairwise distances within B. cincta ranged from 0.024 to 0.072

suggesting the existence of a complex of cryptic species.

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references

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