Antimicrobial activities of novel cultivable bacteria isolated from marine sponge Tedania anhelans

Zhen Zeng; Jing Zhao; Caihuan Ke; Dexiang Wang

doi:10.1007/s00343-013-2223-y

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Antimicrobial activities of novel cultivable bacteria isolated from marine sponge Tedania anhelans

Updated：2023-05-16

- Antimicrobial activities of novel cultivable bacteria isolated from marine sponge Tedania anhelans
- Journal of Oceanology and Limnology Vol. 31, Issue 3, Pages: 581-590(2013)
- Affiliations：
  
  1. State Key Laboratory of Marine Environmental Science Xiamen University
  2. College of Ocean and Earth Sciences Xiamen University
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-2223-y
  CLC：
- Online First：08 May 2013，
  
  Published：2013
- Accepted：
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Zeng, Z., Zhao, J., Ke, C. et al. Antimicrobial activities of novel cultivable bacteria isolated from marine sponge Tedania anhelans, ., Chin. J. Ocean. Limnol. 31, 581–590 (2013). https://doi.org/10.1007/s00343-013-2223-y
DOI：

Zeng, Z., Zhao, J., Ke, C. et al. Antimicrobial activities of novel cultivable bacteria isolated from marine sponge Tedania anhelans, ., Chin. J. Ocean. Limnol. 31, 581–590 (2013). https://doi.org/10.1007/s00343-013-2223-y DOI：

摘要

Abstract

Marine sponge Tedania anhelans distributes throughout the intertidal zone of Fujian

southeastern China

and is a potential source of natural bioactive products. The sponge harbors a large number of bacterial groups that have been identified using various techniques

including fluorescent in situ hybridization (FISH). Fractionation of dissociated sponge allowed isolation of 25 bacterial species. Based on 16S rRNA gene sequencing

phylogenetic analysis attributed most of these eubacteria to α-Proteobacteria

γ-Proteobacteria

Cytophaga / Flavobacterium / Bacteroidetes (CFB group)

and the family Bacillaceae of Gram-positive bacteria. In sequence similarity

five putatively novel species were identified with less than 98% similarity to other strains in the NCBI database. Tests for antimicrobial activities were performed against Gram-positive bacteria

Gram-negative bacteria

fungi

antitumor indicators Escherichia coli 343/591 (with DNA repair deficiency)

regular E. coli 343/636 (with different DNA repair capacity)

and 10 bacterial isolates exhibited inhibitory bioactivities. Among these strains

three isolates were detected involving function gene NRPS-A domains

which were most closely related to the amino acid sequences of linear gramicidin synthetase and pyoverdine synthetase. These results contribute to our knowledge of the microbes associated with marine sponges and further reveal novel bacterial resources for the screening of bioactive marine natural products.

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references

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