Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression

Feng Wang; Haimin Chen; Xiaojun Yan; Yanling Zheng

doi:10.1007/s00343-013-2215-y

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Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression

Updated：2023-05-16

- Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression
- Journal of Oceanology and Limnology Vol. 31, Issue 3, Pages: 560-569(2013)
- Affiliations：
  
  1. Department of Clinical Laboratory Lihuili Hospital of Ningbo Medical Center
  2. Key Laboratory of Applied Marine Biotechnology Ministry of Education Ningbo University
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-2215-y
  CLC：
- Online First：08 May 2013，
  
  Published：2013
- Accepted：
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Wang, F., Chen, H., Yan, X. et al. Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression., Chin. J. Ocean. Limnol. 31, 560–569 (2013). https://doi.org/10.1007/s00343-013-2215-y
DOI：

Wang, F., Chen, H., Yan, X. et al. Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression., Chin. J. Ocean. Limnol. 31, 560–569 (2013). https://doi.org/10.1007/s00343-013-2215-y DOI：

摘要

Abstract

This study investigated the molecular mechanism of anti-tumor effect of fascaplysin

a nitrogenous red pigment firstly isolated from a marine sponge. Microarray analysis show that the TNF and TNF receptor superfamily in human umbilical vein endothelial cells (HUVEC) and human hepatocarcinoma cells (BEL-7402) were significantly regulated by fascaplysin. Western Blot results reveal that fascaplysin increased the expression of cleaved caspase-9

active caspase-3

and decreased the level of procaspase-8 and Bid. Flow cytometry and cytotoxicity tests indicate that fascaplysin sensitized cells to tumor necrosis-related apoptosisinducing ligand-(TRAIL) induced apoptosis

which was markedly blocked by TRAIL R2/Fc chimera

a dominant negative form of TRAIL receptor DR5. Therefore

our results demonstrate that fascaplysin promotes apoptosis through the activation of TRAIL signaling pathway by upregulating DR5 expression.

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references

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