Genetic transformation of marine cyanobacterium Synechococcus sp. CC9311 (Cyanophyceae) by electroporation

Huaxin Chen; Hanzhi Lin; Peng Jiang; Fuchao Li; Song Qin

doi:10.1007/s00343-013-2164-5

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Genetic transformation of marine cyanobacterium Synechococcus sp. CC9311 (Cyanophyceae) by electroporation

Updated：2023-05-16

- Genetic transformation of marine cyanobacterium Synechococcus sp. CC9311 (Cyanophyceae) by electroporation
- Journal of Oceanology and Limnology Vol. 31, Issue 2, Pages: 416-420(2013)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
  3. Yantai Institute of Coastal Zone Research Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-2164-5
  CLC：
- Online First：07 March 2013，
  
  Published：2013
- Accepted：
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Chen, H., Lin, H., Jiang, P. et al. Genetic transformation of marine cyanobacterium Synechococcus sp. CC9311 (Cyanophyceae) by electroporation., Chin. J. Ocean. Limnol. 31, 416–420 (2013). https://doi.org/10.1007/s00343-013-2164-5
DOI：

Chen, H., Lin, H., Jiang, P. et al. Genetic transformation of marine cyanobacterium Synechococcus sp. CC9311 (Cyanophyceae) by electroporation., Chin. J. Ocean. Limnol. 31, 416–420 (2013). https://doi.org/10.1007/s00343-013-2164-5 DOI：

摘要

Abstract

Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin

kanamycin

spectinomycin

and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV

using a kanamycin resistance gene as selectable marker

was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition

the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.

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references

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