Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis

Hongjin Qiao; Xiangquan Liu; Xijia Zhang; Haibin Jiang; Jiying Wang; Limin Zhang

doi:10.1007/s00343-013-2158-3

Your Location：

Home >

Browse articles >

Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis

Updated：2023-05-16

- Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis
- Journal of Oceanology and Limnology Vol. 31, Issue 2, Pages: 391-397(2013)
- Affiliations：
  
  Shandong Marine Fisheries Research Institute Shandong Provincial Key Laboratory of Restoration for Marine Ecology
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-2158-3
  CLC：
- Online First：07 March 2013，
  
  Published：2013
- Accepted：
Scan QR Code
Qiao, H., Liu, X., Zhang, X. et al. Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis., Chin. J. Ocean. Limnol. 31, 391–397 (2013). https://doi.org/10.1007/s00343-013-2158-3
DOI：

Qiao, H., Liu, X., Zhang, X. et al. Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis., Chin. J. Ocean. Limnol. 31, 391–397 (2013). https://doi.org/10.1007/s00343-013-2158-3 DOI：

摘要

Abstract

Amplified fragment length polymorphisms (AFLP) markers were developed to assess the genetic variation of populations and clones of Rhopilema esculentum Kishinouye (Scyphozoa

Rhizostomatidae). One hundred and seventy-nine loci from 56 individuals of two hatchery populations and two wild populations were genotyped with five primer combinations. The polymorphic ratio

Shannon’s diversity index and average heterozygosity were 70.3%

0.346 and 0.228 for the white hatchery population

74.3%

0.313

and 0.201 for the red hatchery population

79.3%

0.349

and 0.224 for the Jiangsu wild population

and 74.9%

0.328 and 0.210 for the Penglai wild population

respectively. Thus

all populations had a relatively high level of genetic diversity. A specific band was identified that could separate the white from the red hatchery population. There was 84.85% genetic differentiation within populations. Individual cluster analysis using unweighted pair-group method with arithmetic mean (UPGMA) suggested that hatchery populations and wild populations could be divided. For the hatchery populations

the white and red populations clustered separately; however

for the wild populations

Penglai and Jiangsu populations clustered together. The genetic diversity at the clone level was also determined. Our data suggest that there are relatively high genetic diversities within populations but low genetic differentiation between populations

which may be related to the long-term use of germplasm resources from Jiangsu Province for artificial seeding and releasing. These findings will benefit the artificial seeding and conservation of the germplasm resources.

关键词

Keywords

references

Chen J, Ding G. 1983. Effect of temperature on the strobilation of jellyfih (Rhopilema esculenta Kishinouye -Scyphozoa, Rhizostomeae). Acta Zool. Sinica, 29: 195–206. (in Chinese with English abstract)

Chen J, Ding G, Liu C. 1984. Effect of light on the strobilation of edible medusa, Rhopilema esculenta Kishinouye (Cnidaria, Scyphozoa). Oceanol. Limnol. Sinica, 15: 310–316. (in Chinese with English abstract)

Custance D. 1967. Studies on strobilation in the Scyphozoa. J. Biol. Educ., 1: 79–81.

Dice L R. 1945. Measures of the amount of ecologic association between species. Ecol., 26: 297–302.

Ding G, Chen J. 1984. The life history of R hopilema esculenta Kishinouye. Journal of Fisheries of China, 15(4): 310–316. (in Chinese with English abstract)

Excoffier L, Laval G, Schneider S. 2005. Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1: 47–50.

Fetip A, Martinez-Rodriguez G, Piferrer F, Carrillo M, Zanuy S. 2000. AFLP analysis confirms exclusive maternal genomic contribution of meiogynogenetic Sea Bass (Dicentrarchus labrax L.). Mar. Biotechnol., 2: 301–306.

Gao X, Cao J, Dong J, Liu X, He C. 2009. AFLP analysis in jellyfish populations of Rhopilema esculentum and Nemopilema nomurai in liaoning coast. Fisheries Science, 28(2): 75–78. (in Chinese with English abstract)

Liu Y, Li J, Ye N. 2011. Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis. Chin. J. Oceanol. Limnol., 29(3): 609–615.

Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. Nat. Acad. Sci., 70(12): 3 321–3 323.

Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89(3): 583–590.

Qu R, Hou L, Lu H, Li H. 2004. The gene flow of population genetic structure. Hereditas (Beijing), 26(3): 377–382.

Rohlf F J. 2000. Ntsys-pc. Numerical Taxonomy and Multivariate Analysis System. Version 2.10e. Applied Biostatics Inc., New York, USA.

Sambrook J, Russell D W. 2002. Molecular Cloning: A Laboratory Manual. 3rd edn. Science Press, Beijing, China. p.6.4. (in Chinese)

Sun G, Liu X, Yang J, Zhang X, Liu A, Tan F. 2010. Genetic diversity of ITS sequences in farmed and natural Rhopilema esculentum populations. Marine Sciences, 34(10): 90–95. (in Chinese with English abstract)

Vos P, Hogers R, Bleeker M, Reijans M, Lee T, Hornes M, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res., 23: 4 072–4 414.

Wright S. 1951. The genetical structure of population. Ann. Eugen., 15: 323–334.

Yeh F C, Yang R C, Boyle T. 1999. POPGENE, version 1.3.1. Microsoft Window-based Freeware for Population Genetic Analysis. University of Alberta, Edmonton, Canada.

You K, Ma C, Gao H, Li F, Zhang M, Qiu Y, Wang B. 2007. Research on the jellyfish (Rhopilema esculentum Kishinouye) and associated aquaculture techniques in China: current status. Aquacult. Int., 15: 479–488.

Zhou Y, Wang Y, Huang M. 2004. Jellyfish, Rhopilema esculenta Kishinouye, enhancement research in the coastal waters of Zhejiang. Journal of Zhejiang Ocean University (Natural Science), 23(1): 28–30. (in Chinese with English abstract)

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits inFenneropenaeus chinensis

Analysis of the genetic diversity and differentiation of Fenneropenaeus penicillatus populations using AFLP technology

Inbreeding and genetic diversity analysis in a hatchery release population and clones of Rhopilema esculentum based on microsatellite markers

Genetic diversity and population structure of the Fujian oyster Crassostrea angulata revealed by mitochondrial COⅠ gene and nuclear gene ITS2 sequences

A preliminary review on population parameters and genetic diversity in Oreochromis mossambicus: meta-regression and haplotype diversity

Related Author

Yue Zhiqin

Wang Weiji

Kong Jie

Dai Jixun

Guiling Zhang

Yuanyu Cao

Zhongbao Li

Jin Chen

Related Institution

Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Yellow Sea Fisheries Research Institute

College of Marine Life Science Ocean University of China

Food Laboratory of qingdao Entry-Exit Inspection and Quarantine Bureau

Institute of Aquaculture Biotechnology Jimei University

Laboratory of Molecular Genetics and Molecular Ecology Fisheries College Jimei University

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰