Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica

Cui Zhao; Cui Liu; Wei Li; Shan Chi; Rongfang Feng; Tao Liu

doi:10.1007/s00343-013-2122-2

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Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica

Updated：2023-05-16

- Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica
- Journal of Oceanology and Limnology Vol. 31, Issue 4, Pages: 830-834(2013)
- Affiliations：
  
  Laboratory of Genetics and Breeding of Marine Organism College of Marine Life Sciences Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-2122-2
  CLC：
- Online First：10 July 2013，
  
  Published：2013
- Accepted：
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Zhao, C., Liu, C., Li, W. et al. Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica, ., Chin. J. Ocean. Limnol. 31, 830–834 (2013). https://doi.org/10.1007/s00343-013-2122-2
DOI：

Zhao, C., Liu, C., Li, W. et al. Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica, ., Chin. J. Ocean. Limnol. 31, 830–834 (2013). https://doi.org/10.1007/s00343-013-2122-2 DOI：

摘要

Abstract

Restriction site amplified polymorphism (RSAP) was used

for the first time

to analyze the genetic structure and diversity of four

mainly cultivated

varieties of the brown alga

Saccharina japonica. Eighty-eight samples from varieties “ Rongfu ”

“ Fujian ”

“ Ailunwan ” and “ Shengchanzhong ” were used for the genetic analyses. One hundred and ninety-eight bands were obtained using eight combinations of primers. One hundred and ninety-one (96.46%) were polymorphic bands. Nei’s genetic diversity was 0.360

and the coefficient of genetic differentiation was 0.357. No inbreeding-type recession was found in the four brown alga varieties and the results of the “ Ailunwan ” variety using samples from 2 years showed that the variety was becoming less diverse during the selection inherent in the breeding program. Genetic diversity and cluster analyses results were consistent with these genetic relationships. The results show the RSAP method is suitable for genetic analysis. Continuous inbreeding and selection could reduce the genetic diversity effectively; therefore periodical supervision is required.

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references

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