Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences

Qinglei Meng; Shi Wang; Lingling Zhang; Xiaoting Huang; Zhenmin Bao

doi:10.1007/s00343-013-2070-x

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Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences

Updated：2023-05-16

- Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences
- Journal of Oceanology and Limnology Vol. 31, Issue 1, Pages: 128-133(2013)
- Affiliations：
  
  1. Key Laboratory of Marine Genetics and Breeding (MGB) Ministry of Education College of Marine Life Sciences Ocean University of China
  2. Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding Freshwater Fisheries Research Institute of Shandong Province
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-2070-x
  CLC：
- Online First：16 January 2013，
  
  Published：2013
- Accepted：
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Meng, Q., Wang, S., Zhang, L. et al. Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences., Chin. J. Ocean. Limnol. 31, 128–133 (2013). https://doi.org/10.1007/s00343-013-2070-x
DOI：

Meng, Q., Wang, S., Zhang, L. et al. Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences., Chin. J. Ocean. Limnol. 31, 128–133 (2013). https://doi.org/10.1007/s00343-013-2070-x DOI：

摘要

Abstract

In situ loop-mediated isothermal amplification (in situ LAMP) combines in situ hybridization and loop-mediated isothermal amplification (LAMP) techniques for chromosomal localization of DNA sequences. In situ LAMP is a method that is generally more specific and sensitive than conventional techniques such as fluorescence in situ hybridization (FISH)

primed in situ labeling (PRINS)

and cycling primed in situ labeling (C-PRINS). Here

we describe the development and application of in situ LAMP to identify the chromosomal localization of DNA sequences. To benchmark this technique

we successfully applied this technique to localize the major ribosomal RNA gene on the chromosomes of the Zhikong scallop (Chlamys farreri).

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Keywords

references

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