Phylogenetic analyses of four species of Ulva and Monostroma grevillei using ITS, rbc L and 18S rDNA sequence data

Zhongheng Lin; Songdong Shen; Weizhou Chen; Huihui Li

doi:10.1007/s00343-013-2069-3

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Phylogenetic analyses of four species of Ulva and Monostroma grevillei using ITS, rbc L and 18S rDNA sequence data

Updated：2023-05-16

- Phylogenetic analyses of four species of Ulva and Monostroma grevillei using ITS, rbc L and 18S rDNA sequence data
- Journal of Oceanology and Limnology Vol. 31, Issue 1, Pages: 97-105(2013)
- Affiliations：
  
  1. Department of Cell Biology School of Biology and Basic Medical Sciences Soochow University
  2. Institute of Marine Biology Shantou University
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-2069-3
  CLC：
- Online First：16 January 2013，
  
  Published：2013
- Accepted：
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Lin, Z., Shen, S., Chen, W. et al. Phylogenetic analyses of four species of Ulva and Monostroma grevillei using ITS, rbc L and 18S rDNA sequence data., Chin. J. Ocean. Limnol. 31, 97–105 (2013). https://doi.org/10.1007/s00343-013-2069-3
DOI：

Lin, Z., Shen, S., Chen, W. et al. Phylogenetic analyses of four species of Ulva and Monostroma grevillei using ITS, rbc L and 18S rDNA sequence data., Chin. J. Ocean. Limnol. 31, 97–105 (2013). https://doi.org/10.1007/s00343-013-2069-3 DOI：

摘要

Abstract

Chlorophyta species are common in the southern and northern coastal areas of China. In recent years

frequent green tide incidents in Chinese coastal waters have raised concerns and attracted the attention of scientists. In this paper

we sequenced the 18S rDNA genes

the internal transcribed spacer (ITS) regions and the rbc L genes in seven organisms and obtained 536–566 bp long ITS sequences

1 377–1 407 bp long rbc L sequences and 1 718–1 761 bp long partial 18S rDNA sequences. The GC base pair content was highest in the ITS regions and lowest in the rbc L genes. The sequencing results showed that the three Ulva prolifera (or U. pertusa) gene sequences from Qingdao and Nan’ao Island were identical. The ITS

18S rDNA and rbc L genes in U. prolifera and U. pertusa from different sea areas in China were unchanged by geographic distance. U. flexuosa had the least evolutionary distance from U. californica in both the ITS regions (0.009) and the 18S rDNA (0.002). These data verified that Ulva and Enteromorpha are not separate genera.

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references

Booton G C, Floyd G L, Fuerst P A. 1998. Polyphyly of tetrasporalean green algae inferred from nuclear small-subunit ribosomal DNA. J. Phycol., 34: 306–311.

Daugbjerg N, Moestrup Ø, Arctander P. 1994. Phylogeny of the genus Pyramimonas (Prasinophyceae, Chlorophyta) inferredfrom the rbcL gene. J. Phycol., 30: 991–999.

Daugbjerg N, Moestrup Ø, Arctander P. 1995. Phylogeny of genera of Prasinophyceae and Pedinophyceae (Chlorophyta) deduced from molecular analysis of the rbcL gene. Phycol. Res., 43: 203–213.

Fletcher R T. 1996. The occurrence of ‘green tide’. In: Schramm W, Nienhuis P H eds. Marine Benthic Vegetation-Recent Changes and the Effects of Eutrophication. Springer Verlag, Berlin. p.7–43.

Friedl T. 1996. Evolution of the polyphyletic genus Pleurastrum (Chlorophyta): inferences from nuclear-encoded ribosomal DNA sequences and motile cell ultrastructure. Phycologia, 35: 456–469.

Hall T A, 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp. Ser., 41: 95–98.

Hayden H S, Blomster J, Maggs C A, Silva P C, Stanhope M J, Waaland J R. 2003. Linnaeus was right all along: Ulva and Enteromorpha are not distinct genera. Eur. J. Phycol., 38: 277–294.

Hayden H S, Waaland J R. 2002. Phylogenetic systematics of the Ulvaceae (Ulvales, Ulvophyceae) using chloroplast and nuclear DNA sequences. J. Phycol., 38: 1 200–1 212.

Jiang P, Wang J F, Cui Y L, Li Y X, Lin H Z, Qin S. 2008. Molecular phylogenetic analysis of attached Ulvaceae species and free-floating Enteromorpha from Qingdao coasts in 2007. Chin. J. Oceanol. Limnol., 26(3): 276–279.

Kudo T, Notoya M. 1999. The free-floating Ulva species growing in the calm seas. Case of the Sea Park of Yokohama City. In: Notoya M ed. Use of Ulva and Restoration of the Environment. Seizando, Tokyo. p.55–70. (in Japanese)

Kumar S, Tamura K, Jakobsen I B, Nei M. 2001. MEGA 2: molecular evolutionary genetics analysis software. Bioinformatics, 17: 1 244–1 245.

Leliaert F, Rousseau F, Reviers De B, Coppejans E. 2003. Phylogeny of the Cladophorophyceae (Chlorophyta) inferred from partial LSU rRNA gene sequences: is the recognition of a separate order Siphonocladales justified? Eur. J. Phycol., 38: 233–246.

Linnaeus C. 1753. Species plantarum, exhibentes plantas rite cognitas, ad genera relatas, cum differentiis specificus, nominibus trivialibus, synonymis selectis, locis natalibus, secundum systema sexuale digestas. Ed. 1. Vols. 1–2, Salvii, Stockholm. Vol. 1, pp [i–xii] 1–560. Vol. 2, pp. [i], 561–1200, [1–30 index].

Li Y Y, Shen S D, He L H, Xu P, Wang G C. 2009. Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis. C hin. J. O ceanol. L imnol., 27(3): 1–9.

Li Y Y, Shen S D, He L H, Xu P, Lu S. 2010. Sequence analysis of rDNA intergenic spacer (IGS) of Porphyra haitanensis. J. Appl. Phycol., 22: 187–193.

Lobban C S, Harrison P J. 1997. Seaweed Ecology and Physiology. Cambridge University Press, Cambridge. 366p.

Manhart J R. 1994. Phylogenetic analysis of green plant rbc L sequences. Mol. Phylogenet. Evol., 3: 114–127.

Malta E J, Draisma S G A, Kamermans P. 1999. Free-floating Ulva in the southwest Netherlands: species or morphotypes? A morphological, molecular and ecological comparison. Eur. J. Phycol., 34: 443–54.

Nozaki H, Ito M, Sano R, Uchida H, Watanabe M M, Takahashi H, Kuroiwa T. 1997a. Phylogenetic analysis of Yamagishiella and Platydorina (Volvocaceae, Chlorophyta) based on rbcL gene sequences. J. Phycol., 33: 272–278.

Nozaki H, Ito M, Watanabe M M, Takahashi H, Kuroiwa T. 1997b. Phylogenetic analysis of morphological species of Carteria (Volvocales, Chlorophyta) based on rbcL gene sequences. J. Phycol., 33: 864–867.

Okamura K. 1921. Illustrations of the Marine Algae of Japan, IV, No. 4. Keigyosha, Tokyo. p.79–83, l.169-70.

Olsen J L, Stam W T, Berger S, Menzel D. 1994. 18S rDNA and evolution in the Dasycladales (Chlorophyta): modern living fossils. J. Phycol., 30: 729–744.

Shimada S, Hiraoka M, Nabata S, Iima M, Masuda M. 2003. Molecular phylogenetic analyses of the Japanese Ulva and Enteromorpha (Ulvales, Ulvophyceae), with special reference to the free-floating Ulva. Phycol. Res., 51: 99–108.

Shimada S, Yokoyama N, Arai S, Hiraoka M. 2008. Phylogeography of the genus Ulva (Ulvophyceae, Chlorophyta), with special reference to the Japanese freshwater and brackish taxa. J. Appl. Phycol., 20: 979–989.

Sun S, Wang F, Li C L, Qin S, Zhou M J, Ding L P, Pang S J, Duan D L, Wang G C, Yin B S, Yu R C, Jiang P, Liu Z L, Zhang G T, Fei X G, Zhou M. 2008. Emerging challenges: massive green algae blooms in the Yellow Sea. Nature Proceedings, hdl: 10101/npre. 2008. 2266.1.

Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, Higgins D G. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids. Res., 24: 4 876–4 882.

Tan I H, Blomster J, Hansen G, Leskinen E, Maggs C A, Mann D G, Sluiman H J, Stanhope M J. 1999. Molecular phylogenetic evidence for a reversible morphogenetic switch controlling the gross morphology of two common genera of green seaweeds, Ulva and Enteromorpha. Mol. Biol. Evol., 16: 1 011–1 018.

van Oppen M J H. 1995 Tracking Trails by Cracking Codes. Ph.D. Dissertation. University of Groningen, Groningen. 166p.

White T J, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innes M A, Gefland D H, Sninsky J J, White T J eds. PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, NY, USA. p.315–322.

Wilcox L W, Lewis L A, Fuerst P A, Floyd G L. 1992. Assessing the relationships of autosporic and zoosporic chlorococcalean green algae with 18S rDNA sequence data. J. Phycol., 28: 381–386.

Yang J, Wang Q, Liu M H, An L J. 1999. A simple method for extracting total DNA of seaweeds. Biotechonl., 9(4): 39–42.

Zechman F W, Theriot E G, Zimmer E A, Chapman R L. 1990. Phylogeny of the Ulvophyceae (Chlorophyta): cladistic analysis of nuclear encoded rRNA sequence data. J. Phycol., 26: 700–710.

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