Sequencing and expression analysis of CD3γ/δ and CD3ɛ chains in mandarin fish, Siniperca chuatsi

Zheng Guo; Pin Nie

doi:10.1007/s00343-013-2018-1

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Sequencing and expression analysis of CD3γ/δ and CD3ɛ chains in mandarin fish, Siniperca chuatsi

Updated：2023-05-16

- Sequencing and expression analysis of CD3γ/δ and CD3ɛ chains in mandarin fish, Siniperca chuatsi
- Journal of Oceanology and Limnology Vol. 31, Issue 1, Pages: 106-117(2013)
- Affiliations：
  
  1. State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-013-2018-1
  CLC：
- Online First：16 January 2013，
  
  Published：2013
- Accepted：
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Guo, Z., Nie, P. Sequencing and expression analysis of CD3γ/δ and CD3ɛ chains in mandarin fish, Siniperca chuatsi, ., Chin. J. Ocean. Limnol. 31, 106–117 (2013). https://doi.org/10.1007/s00343-013-2018-1
DOI：

Guo, Z., Nie, P. Sequencing and expression analysis of CD3γ/δ and CD3ɛ chains in mandarin fish, Siniperca chuatsi, ., Chin. J. Ocean. Limnol. 31, 106–117 (2013). https://doi.org/10.1007/s00343-013-2018-1 DOI：

摘要

Abstract

The genomic and cDNA sequences of the CD3γ/δ and CD3ɛ homologues in the mandarin fish

Siniperca chuats i

were determined. As in other vertebrate CD3 molecules

the deduced amino acid sequences of mandarin fish CD3γ/δ and CD3ɛ contained conserved residues and motifs

such as cysteine residues and CXXC and immunoreceptor tyrosine-based activation motifs. However

mandarin fish CD3γ/δ and CD3ɛ showed some differences to their mammalian counterparts

specifically the absence of a negatively charged residue in the transmembrane region of CD3γ/δ. Additionally

while an N -glycosylation site was present in CD3ɛ

the site was not observed in CD3γ/δ. The CD3γ/δ and CD3ɛ subunit sequences contain six and five exons

respectively

consistent with homologues from Atlantic salmon

Salmo salar. Phylogenetic analysis also revealed that CD3γ/δ and CD3ɛ in mandarin fish are closely related to their counterparts in Acanthopterygian fish. Real-time PCR showed CD3γ/δ and CD3ɛ were expressed mainly in the thymus and spleen in normal healthy fish and

to a lesser extent

in mucosal-associated lymphoid tissues

such as the intestine and gills. When lymphocytes isolated from head kidney were treated with the mitogens phytohemagglutinin

concanavalin

and polyriboinosinic polyribocytidylic acid

mRNA expression levels of CD3γ/δ and CD3ɛ were significantly elevated within 12 h of treatment. This indicated the presence of T lymphocytes in the head kidney of teleost fish

and also the recognition of mitogens by the lymphocytes. Mandarin fish infected with the bacterial pathogen Flavobacterium columnare also showed an increase in the expression of CD3γ/δ and CD3ɛ mRNA

indicating that CD3γ/δ and CD3ɛ lymphocytes are involved in the immune response of this species.

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Keywords

references

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