Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding

Changkao Mu; Shuai Liu; Weiwei Song; Ronghua Li; Chunlin Wang

doi:10.1007/s00343-012-1237-1

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Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding

Updated：2023-05-16

- Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding
- Journal of Oceanology and Limnology Vol. 30, Issue 4, Pages: 638-643(2012)
- Affiliations：
  
  School of Marine Science of Ningbo University
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1237-1
  CLC：
- Online First：02 July 2012，
  
  Published：2012
- Accepted：
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Mu, C., Liu, S., Song, W. et al. Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding., Chin. J. Ocean. Limnol. 30, 638–643 (2012). https://doi.org/10.1007/s00343-012-1237-1
DOI：

Mu, C., Liu, S., Song, W. et al. Enhanced resistance of Portunus trituberculatus to Vibrio alginolyticus by selective breeding., Chin. J. Ocean. Limnol. 30, 638–643 (2012). https://doi.org/10.1007/s00343-012-1237-1 DOI：

摘要

Abstract

We established a line (screened) of Portunus trituberculatus by selectively breeding individuals that survived from challenge with Vibrio alginolyticus

and compared the response of screened and unscreened (control) P. trituberculatus challenged with V. alginolyticus. We measured superoxide dismutase

catalase

acid phosphatase

alkaline phosphatase

and peroxidase activity and the content of hemocyanin in the plasma and phenoloxidase activity in serum. The cumulative survival rate after 24-h challenge with V. alginolyticus was significantly higher in the screened crabs than in the unscreened crabs (P <0.05). T-SOD and PO activity were significantly lower in the screened stock than in the unscreened stock (P <0.05). POD

CAT

and ACP activity and hemocyanin content were significantly higher in the screened stock than in the unscreened stock. Our results suggest that the screened stock was more resistant to infection. Furthermore

the indices we measured may be used to evaluate the health state of P. trituberculatus.

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references

Agius D R, Bannister W H, Balzan R. 1998. Prokaryotic iron superoxide dismutase replaces cytosolic copper, zinc superoxide dismutase in protecting yeast cells against oxidative stress. Biochem. Mol. Biol. Int., 44: 41–49.

Aguirre J, Momberg M R, Hewitt D, Hansberg W. 2005. Reactive oxygen species and development in microbial eukaryotes. Trends Microbiol., 13: 111–118.

Arala-Chaves M, Sequeira T. 2000. Is there any kind of adaptive immunity in invertebrates? Aquaculture, 191: 247–258.

Barnett Y A, King C M. 1995. An investigation of antioxidant status, DNA repair capacity and mutation as a function of age in humans. Mutat. Res., 338: 115–128.

Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–254.

Cerenius L, Söerhäl K K. 2004. The prophenoloxidaseactivating system in invertebrates. Immunol. Rev., 198: 116–126.

Chae H Z, Kim I H, Kim K, Rhee S G. 1993. Cloning, sequencing, and mutation of thiol-specific antioxidant gene of Saccharomyces cerevisiae. J. Biol. Chem., 268: 16 815–16 821.

Chambers J P, Aquino L, Glew R H, Lee R E, McCafferty L R. 1977. Determination of serum acid phosphatase in Gaucher’s disease using 4-methylumbelliferyl phosphate. Clin. Chim. Acta, 80: 67–77.

Chen H T, Xie L P, Yu Z Y, Xu G R, Zhan R Q. 2005. Chemical modification studies on alkaline phosphatase from pearl oyster (Pinctada fucata): a substrate reaction course analysis and involvement of essential arginine and lysine residues at the active site. Int. J. Biochem. Cell B, 37: 1 446–1 457.

Conesa A, Punt P J, van den Hondel C A M. 2002. Fungal peroxidases: molecular aspects and applications. J. Biotechnol., 93: 143–158.

Cong M, Song L S, Wang L L, Zhao J M, Qiu L M, Li L, Zhang H. 2008. The enhanced immune protection of Zhikong scallop Chlamys farreri on the secondary encounter with Listonella anguillarum. Comp. Biochem. Phys. B, 151: 191–196.

De S K, De M, Banerjee R K. 1986. Localization and origin of the intestinal peroxidase—effect of adrenal glucocorticoids J. Steroid Biochem., 24: 629–635.

Downs C A, Fauth J E, Woodley C M, 2001 Assessing the health of grass shrimp (Palaeomonetes pugio) exposed to natural and anthropogenic stressors: a molecular biomarker system. Mar. Biotechnol. (NY), 3: 380–397.

Elsayed N. 2001. Antioxidant mobilization in response to oxidative stress: a dynamic environmental-nutritional interaction. Nutrition, 17: 828–834.

Faulhaber L M, Karp R D. 1992. A diphasic immune response against bacteria in the American cockroach. Immunology, 75: 378–381.

Flajnik M F, Du Pasquier L. 2004. Evolution of innate and adaptive immunity: can we draw a line? Trends Immunol., 25: 640–644.

Fridovich I. 1997. Superoxide anion radical (O −2 ), superoxide dismutases, and related matters. J. Biol. Chem., 272: 18 515–18 517.

Gai Y, Zhao J, Song L, Li C, Zheng P, Qiu L, Ni D. 2008. A prophenoloxidase from the Chinese mitten crab Eriocheir sinensis: gene cloning, expression and activity analysis. Fish Shellfish Immunol., 24: 156–167.

Garba I H, Gatsing D, Ubom G. 2006. Elevated total and isoenzyme forms of acid phosphatase in Falciparum malaria. Comptes Rendus Biologies, 329: 75–78.

Goth L. 1991. A simple method for determination of serum catalase activity and revision of reference range. Clin. Chim. Acta, 196: 143–151.

Hamid M, Khalilur R. 2009. Potential applications of peroxidases. Food Chem., 115: 1 177–1 186.

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