Biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) exposed to in vivo sub-lethal copper concentrations

Yifeng Li; Zhongqi Gu; Hong Liu; Heding Shen; Jinglong Yang

doi:10.1007/s00343-012-1232-6

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Biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) exposed to in vivo sub-lethal copper concentrations

Updated：2023-05-16

- Biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) exposed to in vivo sub-lethal copper concentrations
- Journal of Oceanology and Limnology Vol. 30, Issue 5, Pages: 738-745(2012)
- Affiliations：
  
  1. College of Fisheries and Life Science Shanghai Ocean University
  2. Shengsi Service Center of Marine Science and Technology Development
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1232-6
  CLC：
- Online First：05 September 2012，
  
  Published：2012
- Accepted：
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Li, Y., Gu, Z., Liu, H. et al. Biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) exposed to in vivo sub-lethal copper concentrations., Chin. J. Ocean. Limnol. 30, 738–745 (2012). https://doi.org/10.1007/s00343-012-1232-6
DOI：

Li, Y., Gu, Z., Liu, H. et al. Biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) exposed to in vivo sub-lethal copper concentrations., Chin. J. Ocean. Limnol. 30, 738–745 (2012). https://doi.org/10.1007/s00343-012-1232-6 DOI：

摘要

Abstract

Many aquatic organisms are negatively affected by exposure to high copper concentrations. We investigated the biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) to copper exposure. In vivo bioassays using M. coruscus and different copper concentrations were conducted. The activity of six biomarkers

namely superoxide dismutase (SOD)

catalase (CAT)

acid phosphatase (ACP)

alkaline phosphatase (AKP)

glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) were measured. Survival rates decreased with increased copper concentrations and exposure times. The LC50 values at 48

and 96 h exposure were 0.48

0.37

and 0.32 mg/L

respectively. Within digestive glands

CAT activity increased with increasing Cu concentrations. The activity of AKP showed no significant change

while the remaining four enzymes showed decreasing activity with increasing Cu concentrations. Within the gills

AKP activity increased when the Cu concentration was 0.05 mg/L

but showed no significant changes at higher concentrations. Activity of CAT and ACP within gills tended to decrease with increasing Cu concentration. The activity of SOD and GPT decreased at an exposure concentration of 0.2 mg/L. GOT activity within gills decreased at 0.1 mg/L and increased at an exposure concentration of 0.2 mg/L. Within the adductor muscle

AKP activity increased at 0.05 mg/L but did not change at higher exposure concentrations. ACP activity within adductor muscle tissue showed no change

while activities of CAT

GOT and GPT decreased with increasing Cu concentrations. SOD activity within the adductor muscle tissue significantly decreased at the 0.02

0.05 and 0.2 mg/L exposure concentrations. Our results show tissue specific differences for the six biomarkers in for M. coruscus. Our findings provide the basis for the establishment of reference activity levels against which biomarker changes can be estimated

and are essential preliminary steps in development of in vivo bioassays.

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references

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