Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences

Dongdong Xu; Sanlei Li; Bao Lou; Yurong Zhang; Wei Zhan; Huilai Shi

doi:10.1007/s00343-012-1197-5

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Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences

Updated：2023-05-16

- Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences
- Journal of Oceanology and Limnology Vol. 30, Issue 4, Pages: 604-610(2012)
- Affiliations：
  
  1. Key Laboratory of Mariculture and Enhancement of Zhejiang Province Marine Fishery Institute of Zhejiang Province
  2. College of Marine Science Zhejiang Ocean University
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1197-5
  CLC：
- Online First：02 July 2012，
  
  Published：2012
- Accepted：
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Xu, D., Li, S., Lou, B. et al. Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences., Chin. J. Ocean. Limnol. 30, 604–610 (2012). https://doi.org/10.1007/s00343-012-1197-5
DOI：

Xu, D., Li, S., Lou, B. et al. Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences., Chin. J. Ocean. Limnol. 30, 604–610 (2012). https://doi.org/10.1007/s00343-012-1197-5 DOI：

摘要

Abstract

Japanese flounder is one of the most important commercial species in China; however

information on the genetic background of natural populations in China seas is scarce. The lack of genetic data has hampered fishery management and aquaculture development programs for this species. In the present study

we have analyzed the genetic diversity in natural populations of Japanese flounder sampled from the Yellow Sea (Qingdao population

QD) and East China Sea (Zhoushan population

ZS) using 10 polymorphic microsatellite loci and cytochrome c oxidase subunit I (COI) sequencing data. A total of 68 different alleles were observed over 10 microsatellite loci. The total number of alleles per locus ranged from 2 to 9

and the number of genotypes per locus ranged from 3 to 45. The observed heterozygosity and expected heterozygosity in QD were 0.733 and 0.779

respectively

and in ZS the heterozygosity values were 0.708 and 0.783

respectively. Significant departures from Hardy-Weinberg equilibrium were observed in 7 of the 10 microsatellite loci in each of the two populations. The COI sequencing analysis revealed 25 polymorphic sites and 15 haplotypes in the two populations. The haplotype diversity and nucleotide diversity in the QD population were 0.746±0.072 8 and 0.003 34±0.001 03 respectively

and in ZS population the genetic diversity values were 0.712±0.047 0 and 0.003 18±0.000 49

respectively. The microsatellite data ( F st =0.048 7

P <0.001) and mitochondrial DNA data (F st =0.128

P <0.001) both revealed significant genetic differentiation between the two populations. The information on the genetic variation and differentiation in Japanese flounder obtained in this study could be used to set up suitable guidelines for the management and conservation of this species

as well as for managing artificial selection programs. In future studies

more geographically diverse stocks should be used to obtain a deeper understanding of the population structure of Japanese flounder in the China seas and adjacent regions.

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