Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish

Dongdong Wang; Fuhua Li; Shihao Li; Rong Wen; Jianhai Xiang

doi:10.1007/s00343-012-1195-7

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Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish

Updated：2023-05-16

- Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish
- Journal of Oceanology and Limnology Vol. 30, Issue 4, Pages: 611-619(2012)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1195-7
  CLC：
- Online First：02 July 2012，
  
  Published：2012
- Accepted：
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Wang, D., Li, F., Li, S. et al. Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish., Chin. J. Ocean. Limnol. 30, 611–619 (2012). https://doi.org/10.1007/s00343-012-1195-7
DOI：

Wang, D., Li, F., Li, S. et al. Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish., Chin. J. Ocean. Limnol. 30, 611–619 (2012). https://doi.org/10.1007/s00343-012-1195-7 DOI：

摘要

Abstract

Antimicrobial peptides (AMPs)

as key immune effectors

play important roles in the innate immune system of invertebrates. Different types of AMPs

including Penaeidin

Crustin

ALF (antilipopolysaccharide factor) have been identified in different penaeid shrimp; however

systematic analyses on the function of different AMPs in shrimp responsive to different types of bacteria are very limited. In this study

we analyzed the expression profiles of AMPs in the Chinese shrimps

Fenneropenaeus chinensis

simultaneously by real-time RT-PCR (reverse transcription-polymerase chain reaction) when shrimp were challenged with Micrococcus lysodeikticus (Gram-positive

G+) or Vibrio anguillarium (Gram-negative

G−). Different AMPs showed different expression profiles when shrimp were injected with one type of bacterium

and one AMP also showed different expression profiles when shrimp were challenged with different bacteria. Furthermore

the expression of these AMPs showed temporal expression profiles

suggesting that different AMPs function coordinately in bacteria-infected shrimp. An RNA interference approach was used to study the function of the Relish transcription factor in regulating the transcription of different AMPs. The current study showed that Relish could regulate the transcription of different AMPs in shrimp. Differential expression profiles of AMPs in shrimp injected with different types of bacteria indicated that a complicated antimicrobial response network existed in shrimp. These data contribute to our understanding of immunity in shrimp and may provide a strategy for the control of disease in shrimp.

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references

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