Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum

Xianghai Tang; Rencheng Yu; Mingjiang Zhou; Zhigang Yu

doi:10.1007/s00343-012-1142-7

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Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum

Updated：2023-05-16

- Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum
- Journal of Oceanology and Limnology Vol. 30, Issue 2, Pages: 256-263(2012)
- Affiliations：
  
  1. Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education Ocean University of China
  2. Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1142-7
  CLC：
- Online First：01 March 2012，
  
  Published：2012
- Accepted：
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Tang, X., Yu, R., Zhou, M. et al. Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum, ., Chin. J. Ocean. Limnol. 30, 256–263 (2012). https://doi.org/10.1007/s00343-012-1142-7
DOI：

Tang, X., Yu, R., Zhou, M. et al. Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum, ., Chin. J. Ocean. Limnol. 30, 256–263 (2012). https://doi.org/10.1007/s00343-012-1142-7 DOI：

摘要

Abstract

The dinoflagellate Alexandrium minutum is often associated with harmful algal blooms (HABs). This species consists of many strains that differ in their ability to produce toxins but have similar morphology

making identification difficult. In this study

species-specific rRNA probes were designed for whole-cell fluorescence in situ hybridization (FISH) to distinguish A. minutum from two phylogenetic clades. We acquired the complete SSU to LSU rDNA sequences (GenBank accession numbers JF906989-JF906999) of 11 Alexandrium strains and used these to design rRNA targeted oligonucleotide probes. Three ribotype-specific probes

M-GC-1

M-PC-2

and M-PC-3

were designed. The former is specific for the GC clade (“Global clade”) of A. minutum

the majority of which have been found non-toxic

and the latter two are specific for the PSP (paralytic shellfish poisoning)-producing PC clade (“Pacific clade”). The specificity of these three probes was confirmed by FISH. All cells in observed fields of view were fluorescently labeled when probes and target species were incubated under optimized FISH conditions. However

the accessibility of rRNA molecules in ribosomes varied among the probe binding positions. Thus

there was variation in the distribution of positive signals in labeled cells within nucleolus and cytosol (M-GC-1

M-PC-3)

or just nucleolus (M-PC-2). Our results provide a methodological basis for studying the biogeography and population dynamics of A. minutum

and providing an early warning of toxic HABs.

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references

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