Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5

Jinling Cai; Ying Wei; Yupeng Zhao; Guanghua Pan; Guangce Wang

doi:10.1007/s00343-012-1132-9

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Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5

Updated：2023-05-16

- Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5
- Journal of Oceanology and Limnology Vol. 30, Issue 4, Pages: 620-626(2012)
- Affiliations：
  
  1. Tianjin Key Laboratory of Marine Resources and Chemistry College of Marine Science and Engineering Tianjin University of Science and Technology
  2. Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1132-9
  CLC：
- Online First：02 July 2012，
  
  Published：2012
- Accepted：
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Cai, J., Wei, Y., Zhao, Y. et al. Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5., Chin. J. Ocean. Limnol. 30, 620–626 (2012). https://doi.org/10.1007/s00343-012-1132-9
DOI：

Cai, J., Wei, Y., Zhao, Y. et al. Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5., Chin. J. Ocean. Limnol. 30, 620–626 (2012). https://doi.org/10.1007/s00343-012-1132-9 DOI：

摘要

Abstract

The effects of different NaCl concentrations

nitrogen sources

carbon sources

and carbon to nitrogen molar ratios on biomass accumulation and polyhydroxybutyrate (PHB) production were studied in batch cultures of the marine photosynthetic bacterium Rhodovulum sulfidophilum P5 under aerobic-dark conditions. The results show that the accumulation of PHB in strain P5 is a growth-associated process. Strain P5 had maximum biomass and PHB accumulation at 2%–3% NaCl

suggesting that the bacterium can maintain growth and potentially produce PHB at natural seawater salinity. In the nitrogen source test

the maximum biomass accumulation (8.10±0.09 g/L) and PHB production (1.11±0.13 g/L and 14.62%±2.2 of the cell dry weight) were observed when peptone and ammonium chloride were used as the sole nitrogen source. NH 4 + -N was better for PHB production than other nitrogen sources. In the carbon source test

the maximum biomass concentration (7.65±0.05 g/L) was obtained with malic acid as the sole carbon source

whereas the maximum yield of PHB (5.03±0.18 g/L and 66.93%±1.69% of the cell dry weight) was obtained with sodium pyruvate as the sole carbon source. In the carbon to nitrogen ratios test

sodium pyruvate and ammonium chloride were selected as the carbon and nitrogen sources

respectively. The best carbon to nitrogen molar ratio for biomass accumulation (8.77±0.58 g/L) and PHB production (6.07±0.25 g/L and 69.25%±2.05% of the cell dry weight) was 25. The results provide valuable data on the production of PHB by R. sulfidophilum P5 and further studies are on-going for best cell growth and PHB yield.

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references

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