Molecular identification and culture trials of Eutreptiella gymnastica (Eutreptiales, Euglenophyceae)

Na Xu; Shaojun Pang; Tifeng Shan; Feng Liu; Xiaobo Zhao; Suqin Gao

doi:10.1007/s00343-012-1111-1

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Molecular identification and culture trials of Eutreptiella gymnastica (Eutreptiales, Euglenophyceae)

Updated：2023-05-16

- Molecular identification and culture trials of Eutreptiella gymnastica (Eutreptiales, Euglenophyceae)
- Journal of Oceanology and Limnology Vol. 30, Issue 3, Pages: 446-455(2012)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1111-1
  CLC：
- Online First：18 October 2011，
  
  Published：2012
- Accepted：
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Xu, N., Pang, S., Shan, T. et al. Molecular identification and culture trials of Eutreptiella gymnastica (Eutreptiales, Euglenophyceae)., Chin. J. Ocean. Limnol. 30, 446–455 (2012). https://doi.org/10.1007/s00343-012-1111-1
DOI：

Xu, N., Pang, S., Shan, T. et al. Molecular identification and culture trials of Eutreptiella gymnastica (Eutreptiales, Euglenophyceae)., Chin. J. Ocean. Limnol. 30, 446–455 (2012). https://doi.org/10.1007/s00343-012-1111-1 DOI：

摘要

Abstract

During a survey for marine microalgal resources

we isolated a rare marine euglenoid from the coastal waters of Qingdao

China in 2009

and established a pure culture. Electron microscopic and molecular phylogenetic (18S rDNA and 16S rDNA sequences) analyses revealed a close affinity with Eutreptiella gymnastica

a bloom-forming species. Different culture conditions were monitored to understand optimal E. gymnastica growth characteristics. The optimal growth conditions in a batch culture of this isolate were 20°C

160 μmol photons/(m2·s) of white light

and a salinity of 10–31. Nutrient experiments demonstrated that growth increased dramatically with a phosphorus concentration greater than 72 μmol/L. Understanding the effect of culture conditions on E. gymnastica may help understanding the blooming mechanism of this alga in its natural environment.

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references

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