A new simple screening method for the detection of paralytic shellfish poisoning toxins

Jinping Cheng; Shuaishuai Pi; Shufeng Ye; Haomin Gao; Lei Yao; Zhenyi Jiang; Yuling Song; Lei Xi

doi:10.1007/s00343-012-1097-8

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A new simple screening method for the detection of paralytic shellfish poisoning toxins

Updated：2023-05-16

- A new simple screening method for the detection of paralytic shellfish poisoning toxins
- Journal of Oceanology and Limnology Vol. 30, Issue 5, Pages: 786-790(2012)
- Affiliations：
  
  1. School of Environmental Science and Engineering Shanghai Jiao Tong University
  2. Key Laboratory of Marine Integrated Monitoring and Applied Technologies of Harmful Algal Blooms Chinese State Oceanic Administration
  3. Shanghai Municipal Oceanic Bureau
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1097-8
  CLC：
- Online First：05 September 2012，
  
  Published：2012
- Accepted：
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Cheng, J., Pi, S., Ye, S. et al. A new simple screening method for the detection of paralytic shellfish poisoning toxins., Chin. J. Ocean. Limnol. 30, 786–790 (2012). https://doi.org/10.1007/s00343-012-1097-8
DOI：

Cheng, J., Pi, S., Ye, S. et al. A new simple screening method for the detection of paralytic shellfish poisoning toxins., Chin. J. Ocean. Limnol. 30, 786–790 (2012). https://doi.org/10.1007/s00343-012-1097-8 DOI：

摘要

Abstract

The current testing for paralytic shellfish poisoning (PSP) in shellfish is based on the mouse bioassay (MBA). To alleviate animal welfare concerns

we evaluated the utility of using sublethal indicators of toxicity as an alternative to measuring time to death. Live mice were injected with a PSP congener and the changes in neurotransmitter levels were measured 60

and 120 min after injection. Acetylcholine (ACh) was the most sensitive marker for PSP toxicity. The changes in neurotransmitter levels were most pronounced in the blood. Thus

measurement of Ach levels in the blood may serve as a sensitive predictor for PSP that would not require sacrifice of the mice. This method was relatively simple

sensitive (less than 1 μg/kg weight

equivalent to 20 ng/mL)

low maintenance

and rapid (less than 60 min).

关键词

Keywords

references

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