Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation

Yulin Cui; Peng Jiang; Jinfeng Wang; Fuchao Li; Yingjie Chen; Guoting Zheng; Song Qin

doi:10.1007/s00343-012-1093-z

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Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation

Updated：2023-05-16

- Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation
- Journal of Oceanology and Limnology Vol. 30, Issue 3, Pages: 471-475(2012)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
  3. Key Laboratory of Marine Biotechnology Ningbo University
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1093-z
  CLC：
- Online First：30 June 2011，
  
  Published：2012
- Accepted：
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Cui, Y., Jiang, P., Wang, J. et al. Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation., Chin. J. Ocean. Limnol. 30, 471–475 (2012). https://doi.org/10.1007/s00343-012-1093-z
DOI：

Cui, Y., Jiang, P., Wang, J. et al. Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation., Chin. J. Ocean. Limnol. 30, 471–475 (2012). https://doi.org/10.1007/s00343-012-1093-z DOI：

摘要

Abstract

Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally

Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome of P. subcordiformis using both of the transgenic techniques

with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation of P. subcordiformis

and will improve fundamental research and enlarge application of this alga.

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Keywords

references

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