Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare

Jin Zhang; Hong Zou; Liangfa Wang; Bei Huang; Nan Li; Guitang Wang; Pin Nie

doi:10.1007/s00343-012-1077-z

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Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare

Updated：2023-05-16

- Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare
- Journal of Oceanology and Limnology Vol. 30, Issue 2, Pages: 269-278(2012)
- Affiliations：
  
  1. State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1077-z
  CLC：
- Online First：01 March 2012，
  
  Published：2012
- Accepted：
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Zhang, J., Zou, H., Wang, L. et al. Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare, ., Chin. J. Ocean. Limnol. 30, 269–278 (2012). https://doi.org/10.1007/s00343-012-1077-z
DOI：

Zhang, J., Zou, H., Wang, L. et al. Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare, ., Chin. J. Ocean. Limnol. 30, 269–278 (2012). https://doi.org/10.1007/s00343-012-1077-z DOI：

摘要

Abstract

Flavobacterium columnare

the etiological agent of columnaris disease

is one of the most important and widespread bacterial pathogens of freshwater fish. In this study

we constructed two artificial selectable markers (chloramphenicol and spectinomycin resistance) for gene transfer in F. columnare. These two new artificial selectable markers

which were created by placing the chloramphenicol or spectinomycin resistance gene under the control of the native acs regulatory region of F. columnare

were functional in both F. columnare and Escherichia coli. The integrative/conjugative plasmids constructed by using these markers were introduced into F. columnare G4 via electroporation or conjugation. The integrated plasmid DNA was confirmed by Southern blotting and PCR analysis. These two markers can be employed in future investigations into gene deletion and the pathogenicity of virulence factors in F. columnare.

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references

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