Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis

Zhangfan Chen; Guangce Wang; Jianfeng Niu

doi:10.1007/s00343-012-1060-8

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Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis

Updated：2023-05-16

- Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis
- Journal of Oceanology and Limnology Vol. 30, Issue 1, Pages: 136-145(2012)
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1060-8
  CLC：
- Online First：15 November 2012，
  
  Published：2012
- Accepted：
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Chen, Z., Wang, G. & Niu, J. Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis, ., Chin. J. Ocean. Limnol. 30, 136–145 (2012). https://doi.org/10.1007/s00343-012-1060-8
DOI：

Chen, Z., Wang, G. & Niu, J. Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis, ., Chin. J. Ocean. Limnol. 30, 136–145 (2012). https://doi.org/10.1007/s00343-012-1060-8 DOI：

摘要

Abstract

Cells of Haematococcus pluvialis Flot. et Will were collected in four different growth phases. We quantified the initial and total enzyme activity of ribulose-1

5-bisphosphate carboxylase (Rubisco) in crude extracts

and the relative expression of large-subunit ribulose-1

5-bisphosphate caboxylase / oxygenase (rbcL) mRNA. We measured the ratio of photosynthetic rate to respiration rate (P/R)

maximal effective quantum yield of photosystem II (F v/F m)

electron transport rate (ETR)

actual photochemical efficiency of PSII in the light (PSII)

and non-photochemical quenching (NPQ). Green vegetative cells were found to be in the most active state

with a relatively higher P/R ratio. These cells also displayed the lowest NPQ and the highest F v/F m

ETR

and PSII

indicating the most effective PSII. However

both Rubisco activity and rbcL mRNA expression were the lowest measured. In orange resting cysts with relatively lower P/R and NPQ

Rubisco activity and rbcL expression reached a peak

while F v/F m

ETR

and ΦPSII were the lowest measured. Taking into account the methods of astaxanthin induction used in industry

we suggest that Rubisco may participate in astaxanthin accumulation in H. pluvialis. A continuous and sufficient supply of a carbon source such as CO2 may therefore aid the large scale production of astaxanthin.

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references

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