Differences in MITF gene expression and histology between albino and normal sea cucumbers (Apostichopus japonicus Selenka)

Heling Zhao; Hongsheng Yang; Huan Zhao; Shilin Liu; Tianming Wang

doi:10.1007/s00343-012-1043-9

Your Location：

Home >

Browse articles >

Differences in MITF gene expression and histology between albino and normal sea cucumbers (Apostichopus japonicus Selenka)

Updated：2023-05-16

- Differences in MITF gene expression and histology between albino and normal sea cucumbers (Apostichopus japonicus Selenka)
- Journal of Oceanology and Limnology Vol. 30, Issue 1, Pages: 80-91(2012)
- Affiliations：
  
  1. Graduate University of Chinese Academy of Sciences
  2. Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1043-9
  CLC：
- Online First：15 November 2012，
  
  Published：2012
- Accepted：
Scan QR Code
Zhao, H., Yang, H., Zhao, H. et al. Differences in MITF gene expression and histology between albino and normal sea cucumbers (Apostichopus japonicus Selenka)., Chin. J. Ocean. Limnol. 30, 80–91 (2012). https://doi.org/10.1007/s00343-012-1043-9
DOI：

Zhao, H., Yang, H., Zhao, H. et al. Differences in MITF gene expression and histology between albino and normal sea cucumbers (Apostichopus japonicus Selenka)., Chin. J. Ocean. Limnol. 30, 80–91 (2012). https://doi.org/10.1007/s00343-012-1043-9 DOI：

摘要

Abstract

Albino Apostichopus japonicus occur both in the wild and in captivity. The offspring of albino A. japonicus also suffer from albinism. The formation of melanin in the melanocytes is dependant on microphthalmia-associated transcription factor (MITF). To investigate the role of MITF in controlling albinism

we cloned the full-length MITF cDNA from A. japonicus and compared MITF mRNA expression in albino and normal A. japonicus. In addition

we used light and electron microscopy to compare histological samples of normal and albino A. japonicus. The body wall of albino adults was characterized by significantly lower levels of MITF expression and lower numbers of epidermal melanocytes

which also contained less melanin. In albino juvenile offspring

MITF expression levels were significantly lower 32 d after fertilization and there were fewer

and less developed

epidermal melanocytes. Thus

we conclude that albino A. japonicus have fewer melanocytes and a reduced ability to synthesize melanin

likely because of lower expression of MITF.

关键词

Keywords

references

Aberdam E, Bertolotto C, Sviderskaya E V, Thillot V, Hemesath T J, Fisher D E, Bennett D C, Ortonne J P, Ballotti R. 1998. Involvement of microphthalmia in the inhibition of melanocyte lineage differentiation and of melanogenesis by agouti signal protein. J. Biol. Chem., 273: 19 560–19 565.

Amiel J, Watkin P M, Tassabehji M, Read A P, Winter R M. 1998. Mutation of the MITF gene in albinism-deafness syndrome (Tietz syndrome). Clin. Dysmorphol., 7: 17–20.

Arthur J K, Kathleen A T, John J G. 2005. Effect of sunlight intensity and albinism on the covering response of the Caribbean sea urchin Tripneustes ventricosus. Mar. Biol., 146: 1 111–1 117.

Barbosa A J A, Castro L P F, Margarida A, Nogueira M F. 1984. A simple and economical modification of the masson-fontana method for staining melanin granules and enterochromaffin cells. Stain Tech., 59: 193–196.

Colin R G. 2007. Melanocytes: The new Black. Int. J. Biochem. Cell Biol., 39: 275–279.

Fukuzawa T, Ide H. 1986. Further studies on the melanophores of periodic albino mutant of Xenopus laevis. J. Embryol. Exp. Morph., 91: 65–78.

Gaitanis G, Chasapi V, Velegraki A. 2005. Novel application of the Masson-Fontana stain for demonstrating Malassezia species melanin-like pigment production in vitro and in clinical specimens. J. Clin. Microbiol., 43: 4 147–4 151.

Guo H, Huang B, Qi F, Zhang S. 2007. Distribution and ultrastructure of pigment cells in the skins of normal and albino adult turbot, Scophthalmus maximus. Chin. J. Oceanol. Limnol., 25: 199–208.

Hyman L H. 1955. The Invertebrates: Echinodermata. McGraw-Hill Press, New York.

Ichiro Y, Kosuke E, Shigeru S, Reiko T, Hiroshi W, Shigeki S, Takaharu N, Kazuho I, Takashi G, Colin R G, Hiroaki Y. 2003. Cloning and functional analysis of ascidian Mitf in vivo: insights into the origin of vertebrate pigment cells. Mech. Dev., 120: 1 489–1 504.

Jiri V, Jan B. 2010. “Transcription physiology” of pigment formation in melanocytes: central role of MITF. Exp. Derm., 19: 617–627.

Kazuhisa T, Clifford T, Ichiro K, Atsushi W, Yoshitaka N, David E F, Masayoshi T. 2000. Ser298 of MITF, a mutation site in Waardenburg syndrome type 2, is a phosphorylation site with functional significance. Hum. Mol. Genet., 9: 125–132.

Levy C, Khaled M, Fisher D E. 2006. MITF: master regulator of melanocyte development and melanoma oncogene. Trends Mol. Med., 12: 406–414.

Nakayama A, Nguyen M T, Chen C C, Opdecamp K, Hodgkinson C A, Arnheiter H. 1998. Mutations in microphthalmia, the mouse homolog of the human deafness gene MITF, affect neuroepithelial and neural crest-derived melanocytes differently. Mech. Dev., 70: 155–166.

Nolan M R, Robert S Jr. 1990. Autosomal albinism affects immunocompetence in the chicken. Devel. Comp. Immunol., 14: 105–112.

Opdecamp K, Nakayama A, Nguyen M T, Hodgkinson C A, Pavan W J, Arnheiter H. 1997. Melanocyte development in vivo and in neural crest cell cultures: crucial dependence on the Mitf basic-helix-loop-helix-zipper transcription factor. Devel., 124: 2 377–2 386.

Seldenrijk R, Huijsman K G H, Heussen A M A, Vandeveerdonk F C G. 1982. A comparative ultrastructural and physiological study on melanophores of wild-type and periodic albino mutants of xenopus-laevis. Cell Tissue Res., 222: 1–9.

Spritz R A, Chiang P W, Oiso N, Alkhateeb A. 2003. Human and mouse disorders of pigmentation. Curr. Opin. Gen. Devel., 13: 284–289.

Takeda K, Yasumoto K, Takada R, Takada S, Watanabe K, Udono T, Saito H, Takahashi K, Shibahara S. 2000. Induction of melanocyte-specific microphthalmia-associated transcription factor by Wnt-3a. J. Biol. Chem., 275: 14 013–14 016.

Tassabehji M, Newton V E, Read A P. 1994. Waardenburg syndrome type 2 caused by mutations in the human microphthalmia (MITF) gene. Nature Genet., 8: 251–255.

Tassabehji M, Newton V E, Liu X Z, Brady A, Donnai D, Krajewska-Walasek M, Murday V, Norman A, Obersztyn E, Rice J C. 1995. The mutational spectrum in Waardenburg syndrome. Hum. Mol. Genet., 4: 2 131–2 137.

Wang J, Hou L, Zhang R, Zhao X, Jiang L, Sun W, An J, Li X. 2007. The tyrosinase gene family and albinism in fish. Chin. J. Oceanol. Limnol., 25: 191–198.

Wasmeier C, Hume A N, Bolasco G, Seabra M C. 2008. Melanosomes at a glance. J. Cell. Sci., 121: 3 995–3 999.

William S O. 2000. The tyrosinase gene and Oculocutaneous Albinism Type 1 (OCA1): a model for understanding the molecular biology of melanin formation. Pigm. Cell. Res., 13: 320–325.

Yang H, Yuan X, Zhou Y, Mao Y, Zhang T, Liu Y. 2005. Effects of body size and water temperature on food consumption and growth in the sea cucumber Apostichopus japonicus (Selenka) with special reference to aestivation. Aquac. Res., 36: 1 085–1 092.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Multiomics provided insights into the molecular mechanisms underlying black-colored mantle in scallops

UPLC-MS metabolomics provides insights into the differences between black- and white-shelled Pacific oysters Crassostrea gigas

Distribution and ultrastructure of pigment cells in the skins of normal and albino adult turbot, Scophthalmus maximus

Screen and effect analysis of immunostimulants for sea cucumber, Apostichopus japonicus

Studies on Distribution Characteristics of CurrentDensity on Anode Wall of Ion Thruster

Related Author

Caihui WANG

Fukai WANG

Peican ZHU

Junlin SONG

Min CHEN

Junhao NING

Xia LU

Chunde WANG

Related Institution

Chinese Academy of Fishery Sciences

Qingdao Agricultural University

School of Agriculture, Ludong University

Ocean University of China

The First Institute of Oceanography SOA

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰