Acclimation-dependent expression of heat shock protein 70 in Pacific abalone (Haliotis discus hannai Ino) and its acute response to thermal exposure

Jiaqi Li; Qingguo He; Hui Sun; Xiao Liu

doi:10.1007/s00343-012-1026-x

Your Location：

Home >

Browse articles >

Acclimation-dependent expression of heat shock protein 70 in Pacific abalone (Haliotis discus hannai Ino) and its acute response to thermal exposure

Updated：2023-05-16

- Acclimation-dependent expression of heat shock protein 70 in Pacific abalone (Haliotis discus hannai Ino) and its acute response to thermal exposure
- Journal of Oceanology and Limnology Vol. 30, Issue 1, Pages: 146-151(2012)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-012-1026-x
  CLC：
- Online First：15 November 2012，
  
  Published：2012
- Accepted：
Scan QR Code
Li, J., He, Q., Sun, H. et al. Acclimation-dependent expression of heat shock protein 70 in Pacific abalone (Haliotis discus hannai Ino) and its acute response to thermal exposure., Chin. J. Ocean. Limnol. 30, 146–151 (2012). https://doi.org/10.1007/s00343-012-1026-x
DOI：

Li, J., He, Q., Sun, H. et al. Acclimation-dependent expression of heat shock protein 70 in Pacific abalone (Haliotis discus hannai Ino) and its acute response to thermal exposure., Chin. J. Ocean. Limnol. 30, 146–151 (2012). https://doi.org/10.1007/s00343-012-1026-x DOI：

摘要

Abstract

Heat shock protein 70 (Hsp70) is one important member of heat shock protein (Hsp) family that is responsible for various stresses

especially thermal stress. Here we examined the response of Hsp70 gene to both chronic and acute thermal exposure in Pacific abalone (Haliotis discus hannai Ino). For the chronic exposure

abalones were maintained at 8

and 30°C for four months and their mRNA levels were measured. The highest mRNA level of Hsp70 gene relative to actin gene was detected in the 30°C-acclimated group

followed by the 8°C-acclimated group and then the 12°C- and 20°C-acclimated groups. After the long-term acclimation

gills from each of the above acclimation groups were dissected and exposed to different temperatures between 8°C and 38°C for 30 min. Hsp70 expression in gills acclimated to different temperatures responded differentially to the same temperature exposure. The incubation temperature that induced maximum Hsp70 mRNA expression was higher in the higher temperature acclimation groups than lower temperature groups. Pacific abalones could alter the expression pattern of Hsp70 gene according to environmental thermal conditions

through which they deal with the stress of thermal variations.

关键词

Keywords

references

Boutet I, Tanguy A, Rousseau S, Auffret M, Moraga D. 2003. Molecular identification and expression of heat shock cognate 70 (HSC70) and heat shock protein 70 (HSP70) genes in the Pacific oyster Crassostrea gigas. Cell Stress Chaperones, 8(1): 76–85.

Cheng P Z, Liu X, Zhang G F, He J G. 2007. Cloning and expression analysis of a HSP70 gene from Pacific abalone (Haliotis discus hannai). Fish & Shellfish Immunology, 22(1–2): 77–87.

Farcy E, Serpentini A, Fievet B, Lebel J M. 2007. Identification of cDNAs encoding HSP70 and HSP90 in the abalone Haliotis tuberculata: Transcriptional induction in response to thermal stress in hemocyte primary culture. Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology, 146(4): 540–550.

Feder M E, Krebs R A. 1998. Natural and genetic engineering of the heat-shock protein Hsp70 in Drosophila melanogaster: Consequences for thermotolerance. Am. Zool., 38(3): 503–517.

Feder M E, Hofmann G E. 1999. Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology. Annu. Rev. Physiol., 61: 243–282.

Franzellitti S, Fabbri E. 2005. Differential HSP70 gene expression in the Mediterranean mussel exposed to various stressors. Biochem. Bioph. Res. Co., 336(4): 1 157–1 163.

Gao Q, Song L S, Ni D J, Wu L T, Zhang H, Chang Y Q. 2007. cDNA cloning and mRNA expression of heat shock protein 90 gene in the haemocytes of Zhikong scallop Chlamys farreri. Comp. Biochem. Physiol. B: Biochem. Mol. Biol., 147(4): 704–715.

Gething M J, Sambrook J. 1992. Protein folding in the cell. Nature, 355(6355): 33–45.

Hara M, Sekino M. 2005. Genetic difference between Ezo-awabi Haliotis discus hannai and Kuro-awabi H. discus discus populations: Microsatellite-based population analysis in Japanese abalone. Fisheries Science, 71(4): 754–766.

Hartl F U. 1996. Molecular chaperones in cellular protein folding. Nature, 381(6583): 571–580.

Hartl F U, Hayer-Hartl M. 2002. Protein folding — Molecular chaperones in the cytosol: from nascent chain to folded protein. Science, 295(5561): 1 852–1 858.

Institute S. 1990. SAS/STAT User’s Guide, Version 6, 4th edition. SAS Institute, Cary, North Carolina.

Kikuchi S, Uki N. 1974. Technical study on artificial spawning of abalone, genus Haliotis I. Relation between water temperature and advancing sexual maturity of Haliotis discus hannai Ino. Bull. Tohuku Reg. Fis. Res. Lab, 33: 69–78.

Lv D H. 1978. A study on the Haliotidae from the coast of China. Studia Marina Sinic, 14: 89–100.

Mayer M P, Bukau B. 2005. Hsp70 chaperones: Cellular functions and molecular mechanism. Cellular and Molecular Life Sciences, 62(6): 670–684.

Nie Z Q, Wang S P. 2004. The status of abalone culture in China. Journal of Shellfish Research, 23(4): 941–945.

Osovitz C J, Hofmann G E. 2005. Thermal history-dependent expression of the Hsp70 gene in purple sea urchins: Biogeographic patterns and the effect of temperature acclimation. J. Exp. Mar. Biol. Ecol., 327(2): 134–143.

Rathinam A V, Chen T T, Grossfeld R M. 2000. Cloning and sequence analysis of a cDNA for an inducible 70 kDa heat shock protein (Hsp70) of the American oyster (Crassostrea virginica). DNA Sequence, 11(3–4): 261–264.

Snyder M J, Girvetz E, Mulder E P. 2001. Induction of marine mollusc stress proteins by chemical or physical stress. Arch. Environ. Contam. Toxicol., 41(1): 22–29.

Ruan W J, Lai M D. 2007. Actin, a reliable marker of internal control? Clinica Chimica Acta, 385: 1–5.

Song L S, Wu L T, Ni D J, Chang Y Q, Xu W, Xing K Z. 2006. The cDNA cloning and mRNA expression of heat shock protein 70 gene in the haemocytes of bay scallop (Argopecten irradians, Lamarck 1819) responding to bacteria challenge and naphthalin stress. Fish & Shellfish Immunology, 21(4): 335–345.

Takle H, Baeverfjord G, Lunde M, Kolstad K, Andersen O. 2005. The effect of heat and cold exposure on HSP70 expression and development of deformities during embryogenesis of Atlantic salmon (Salmo salar). Aquaculture, 249(1–4): 515–524.

Vosloo D, Vosloo A. 2010. Response of cold-acclimated, farmed South African abalone (Haliotis midae) to short-term and long-term changes in temperature. J. Therm. Biol., 35(7): 317–323.

Zhang G F, Que H Y, Liu X, Xu H S. 2004. Abalone mariculture in China. Journal of Shellfish Research, 23(4): 947–950.

Zhang Q Z, Wu X Z, Gao J S, Pan J P. 2003. Cloning, Southern blotting and RT-PCR analysis of a cDNA fragment encoding of 70 kDa heat shock protein (Hsc70) from the oyster (Crassostrea ariakensis). Acta Zool. Sin., 49(5): 708–712.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Analysis of the redox environment and genesis of surface sediment in the northeast Indian Ocean at the Ninety-East Ridge

Integrated biomarker response to assess toxic impacts of iron and manganese on deep-sea mussel Gigantidas platifrons under a deep-sea mining activity scenario

Tracing the settlement region of massive floating green algae in the Yellow Sea

Crack Growth Rate Model Based on Modal Frequencyfor a V-Notched Beam

Study on Temperature Field Characteristics of2.5D Braided Composites

Related Author

Zhourong CAI

Qianru HUANG

Zhengxin YIN

Xin SUI

Sibo LÜ

Meng TANG

Juan PENG

Li ZHOU

Related Institution

Key Laboratory of Marine Environmental Survey Technology and Application, Ministry of Natural Resources

South China Sea Marine Survey Center, Ministry of Natural Resources

Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering

School of Marine Sciences, Sun Yat-sen University

University of Chinese Academy of Sciences

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰