Submesoscale characteristics and transcription of a fatty acid elongase gene from a freshwater green microalgae, Myrmecia incisa Reisigl

Shuiyan Yu; Shicheng Liu; Chunyang Li; Zhigang Zhou

doi:10.1007/s00343-011-9959-z

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Submesoscale characteristics and transcription of a fatty acid elongase gene from a freshwater green microalgae, Myrmecia incisa Reisigl

Updated：2023-05-16

- Submesoscale characteristics and transcription of a fatty acid elongase gene from a freshwater green microalgae, Myrmecia incisa Reisigl
- Journal of Oceanology and Limnology Vol. 29, Issue 1, Pages: 87-95(2011)
- Affiliations：
  
  Key Laboratory of Genetic Resources and Ecology in Aquaculture and Aquaculture E-Institute of Shanghai Municipal Education Commission Shanghai Ocean University
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-9959-z
  CLC：
- Online First：15 January 2011，
  
  Published：2011
- Accepted：
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Yu, S., Liu, S., Li, C. et al. Submesoscale characteristics and transcription of a fatty acid elongase gene from a freshwater green microalgae, Myrmecia incisa Reisigl., Chin. J. Ocean. Limnol. 29, 87–95 (2011). https://doi.org/10.1007/s00343-011-9959-z
DOI：

Yu, S., Liu, S., Li, C. et al. Submesoscale characteristics and transcription of a fatty acid elongase gene from a freshwater green microalgae, Myrmecia incisa Reisigl., Chin. J. Ocean. Limnol. 29, 87–95 (2011). https://doi.org/10.1007/s00343-011-9959-z DOI：

摘要

Abstract

Myrmecia incisa is a green coccoid freshwater microalgae

which is rich in arachidonic acid (ArA

C20: 4ω-6

δ5

14)

a long chain polyunsaturated fatty acid (PUFA)

especially under nitrogen starvation stress. A cDNA library of M. incisa was constructed with λ phage vectors and a 545 nt expressed sequence tag (EST) was screened from this library as a putative elongase gene due to its 56% and 49% identity to Marchantia polymorpha L. and Ostreococcus tauri Courties et Chrétiennot-Dinet

respectively. Based upon this EST sequence

an elongase gene designated MiFAE was isolated from M. incisa via 5′/3′ rapid amplification of cDNA ends (RACE). The cDNA sequence was 1 331 bp long and included a 33 bp 5′-untranslated region (UTR) and a 431 bp 3′-UTR with a typical poly-A tail. The 867 bp ORF encoded a predicted protein of 288 amino acids. This protein was characterized by a conserved histidine-rich box and a MYxYY motif that was present in other members of the elongase family. The genomic DNA sequence of MiFAE was found to be interrupted by three introns with splicing sites of Introns I (81 bp)

II (81 bp)

and III (67 bp) that conformed to the GT-AG rule. Quantitative real-time PCR showed that the transcription level of MiFAE in this microalga under nitrogen starvation was higher than that under normal condition. Prior to the ArA content accumulation

the transcription of MiFAE was enhanced

suggesting that it was possibly responsible for the ArA accumulation in this microalga cultured under nitrogen starvation conditions.

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references

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