Insights into the phylogeny of sporadotrichid ciliates (Protozoa, Ciliophora: Hypotricha) based on genealogical analyses of multiple molecular markers

Xiaoyan Hu; Xiaozhong Hu; Khaled A. S. Al-Rasheid; Saleh A. Al-Farraj; Weibo Song

doi:10.1007/s00343-011-9008-y

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Insights into the phylogeny of sporadotrichid ciliates (Protozoa, Ciliophora: Hypotricha) based on genealogical analyses of multiple molecular markers

Updated：2023-05-16

- Insights into the phylogeny of sporadotrichid ciliates (Protozoa, Ciliophora: Hypotricha) based on genealogical analyses of multiple molecular markers
- Journal of Oceanology and Limnology Vol. 29, Issue 1, Pages: 96-102(2011)
- Affiliations：
  
  1. Laboratory of Protozoology Institute of Evolution and Marine Biodiversity Ocean University of China
  2. Department of Zoology Natural History Museum
  3. Zoology Department King Saud University
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-9008-y
  CLC：
- Online First：15 January 2011，
  
  Published：2011
- Accepted：
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Hu, X., Hu, X., Al-Rasheid, K.A.S. et al. Insights into the phylogeny of sporadotrichid ciliates (Protozoa, Ciliophora: Hypotricha) based on genealogical analyses of multiple molecular markers., Chin. J. Ocean. Limnol. 29, 96–102 (2011). https://doi.org/10.1007/s00343-011-9008-y
DOI：

Hu, X., Hu, X., Al-Rasheid, K.A.S. et al. Insights into the phylogeny of sporadotrichid ciliates (Protozoa, Ciliophora: Hypotricha) based on genealogical analyses of multiple molecular markers., Chin. J. Ocean. Limnol. 29, 96–102 (2011). https://doi.org/10.1007/s00343-011-9008-y DOI：

摘要

Abstract

The sporadotrichid ciliates are an especially diverse group. A number of investigators have studied the morphological

morphogenetic

and molecular relationships among members of this group. Despite this

a consistent classification is still lacking and several important questions about the phylogenetic relationships within this group remain unsolved. To improve our understanding of these relationships

we constructed phylogenetic trees using the nucleotide sequences of the small-subunit rRNA (SSrRNA) gene and amino acid sequences of actin I and α-tubulin. Analyses of SSrRNA gene sequences indicated that: 1) the Sporadotrichida sensu Lynn (2008) and the Oxytrichidae are polyphyletic; 2) the Uroleptus species

which are classified to urostylids

formed a sister group with the oxytrichids; 3) Halteria grandinella

which is grouped morphologically with oligotrich species

clustered within the oxytrichids. These results are congruent with previous studies based on SSrRNA gene sequences. However

the amino acid sequences of actin I and α-tubulin yielded different topologies. The main results are: 1) in all phylogenetic trees

the genus Oxytricha was paraphyletic; 2) Uroleptus was sister to a subset of Urostyla and Holosticha

albeit with low supporting values; 3) Halteria grandinella was separated distantly from the Oxytrichidae in trees inferred from actin I amino acid sequences but clustered with oligotrichids in the α-tubulin analysis. The inconsistency among the trees inferred from these different molecular markers may be caused by rapidly accumulated genetic characterizations of ciliates. Further studies with additional molecular markers and sampling of more taxa are expected to better address the relationships among sporadotrichids.

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references

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