Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

Sufen Zhao; Peimin He

doi:10.1007/s00343-011-1032-4

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Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

Updated：2023-05-16

- Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China
- Journal of Oceanology and Limnology Vol. 29, Issue 6, Pages: 1287-1296(2011)
- Affiliations：
  
  1. College of Fisheries and Life Science Shanghai Ocean University
  2. Fisheries College Guangdong Ocean University
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-1032-4
  CLC：
- Online First：25 October 2011，
  
  Published：2011
- Accepted：
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Zhao, S., He, P. Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China., Chin. J. Ocean. Limnol. 29, 1287–1296 (2011). https://doi.org/10.1007/s00343-011-1032-4
DOI：

Zhao, S., He, P. Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China., Chin. J. Ocean. Limnol. 29, 1287–1296 (2011). https://doi.org/10.1007/s00343-011-1032-4 DOI：

摘要

Abstract

The systematic classification of the Eucheumatoideae is difficult because of their variable morphology and interpretation of reproductive structures. Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam

the Philippines and Indonesia. Combined with morphological characteristics

all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences. The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods. The results indicate that different ITS sequence lengths occurred in the different genera and species. An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma. The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate. Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%–63.90%. All nucleotide variations occurred in the ITS1 and ITS2 regions except for five nucleotide transversions in the 5.8S rDNA region. In addition

the difference was at the branches among congeneric species. Kappaphycus sp. had branches with small buds

while K. alvarezii did not have such a feature. The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was <1.20%. Eucheumatoideae algae cultivated in China consisted of three clades

K. alvarezii

Kappaphycus sp.

and E. denticulatum. The results indicate that ITS sequence analysis was an effective way for identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae.

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references

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